pdf Data Sheet Index

HILIC General Information and references
HILIC of Glucosinolates
HILIC of Glycopeptides
HILIC Glycopeptide Tryptic Mapping , LC-MS
HILIC Metabolomics Profiling
HILIC Metabolomics Comparison to RPC (Tolstikov paper).
HILIC-CEX of Phosphorylation Variants of Histone H1.1
HILIC Small Molecule applications
Comparison of PolyHYDROXYETHYL A® to Amide-80® for small molecule HILIC LC-MS analysis of fermentation broth.
HILIC for small polar acids, nucleic acid bases and nucleosides. A Comparison (Y. Guo & S. Gaiki, J. Chromatogr. A, 1074 (2005) 71-80.) of Amide, Amine, Silica and betain sulfonate functional groups.
HILIC, even on raw silica, Increases LC-MS Sensitivity. LCGC article: " Hydrophilic Interaction Chromatography Using Silica Columns for the Retention of Polar Analytes [small molecules sic] and Enhanced ESI-MS Sensitivity"
HILIC of Underivatized Amino Acids from blood as indicators of tissue transplant rejection.
HILIC of Underivatized Amino Acids from plant extracts.
SPE HILIC micro-SPE tips
SPE High Capacity HILIC micro-SPE tips
Stylus tips Method Development using HILIC SPE tips

SeQuant® AB, ZIC®-HILIC Columns:
"An HPLC Method for the Determination of Pharmaceutical Salts", LCGC AUG-06: Brian W. Pack and Donald S. Risley
Poster: Betain sulfonates bound to silica for Zwitterionic HILIC separations
Zwitterionic HILIC of cationic peptides
Zwitterion HILIC of morphine and glucuronated metabolites
Zwitterion HILIC orthogonal method to RPC
Zwitterionic HILIC of tryptic peptides
Zwitterionic HILIC of homocysteine, methylmalonic acid and succinic acid from biological fluids

HILIC Review Article: Petrus Hemstrom and Knut Irgum, J. Sep. Sci., 2006, 29, 1784 - 1821. "Hydrophilic Interaction Chromatography."

Ion Exchange HPLC of Peptides & Proteins

Monoclonal Antibody Analysis by WCX pdf
Protein Variant Analysis of HgH by WCX (1.8MB pdf).
Intact Protein Isolation for Proteomics Bulletin 2006
Strong Cation Exchange (SCX) of Peptides General Protocol pdf
SCX precolumn fractionation for LC-MS post ICAT™ fraction of peptides for Proteomics
Use of pH and salt gradients for 2-D, Nano-SCX/RPC-LC/MSMS proteomics fractionation of tryptic digests.
HILIC-SCX of Lung Surfactant Proteins from excess lipids.
Weak Cation Exchange of Peptides Using Decreasing pH Gradients pdf
Effect of Organic Solvent on WCX of Proteins pdf

Micro-SPE (MiniSpin columns, 96-Well Spin plates, MicroTip, PrepTip®, Amino Acid Specific Resins)

Cerex System-96 for positive pressure SPE
IMAC General Instructions
MiniSpin General Information and Operating Instructions for HILIC, RPC, SCX, SEC, Unbonded Silica, WAX, or WCX MiniSpin products.
MicroTip.pdf format of UltraMicroSpin columns.
PAGE*Eraser™ micro filters remove particulates from in-gel digests to prolong capillary LC-MS column life.
Spin-Con microDialysis and ultra-filtration for 1-5µl samples
Ultra-Micro PrepTip® RPC General Instructions
Stylus.pdf

Peptides Containing Specific Amino Acids (BioMolecular Technologies Kits)

Pi³MET manual

Pi³TRP manual

Pi³MET-TRP

PhosphoTryosine (or pSer, pThr and pTyr as a group) Selective resins. PhosphoTryosine-QE Kit is Now Available for Delivery
Pi³TYR-phosphate data from 2002 poster on the tyrosine-phosphate specific kit (2.1MB pdf).
Pi³TYR-phosphate-QE ABRF 2003 Poster data pdf (2MB)

Reversed Phase (RPC) Peptide, Protein & Small Molecule HPLC

General HPLC Column Care Information.
Higgins Analytical® HAISIL™ Products.
Catalogs of HAISIL® HPLC Columns, Short Direct Connect Narrowbore (Sprite), Guard/Trap (Piccolo), and/or Cartridge (HAIPEEK) Products.
Complete (2,668K pdf) or individual sections:
(Type A Silica)
HAISIL 100® (small pore) products
Kromasil® 100 small pore columns
HAISIL 300® (wide pore) products.
(Type B Silica)
HAISIL TS Targa ® columns or LC-MS/MS Capillary (Capellini™), Guard/Trap 0.3 - 1mmID (Piccolo™) or Direct Connect Ballistic Gradient, 2mmID (Sprite™) configurations.
HAISIL CS Clipeus® columns or LC-MS/MS Capillary (Capellini™), Guard/Trap 0.3 - 1mmID (Piccolo™) or Direct Connect Ballistic Gradient, 2mmID (Sprite™) configurations.
HAISIL HL High Load columns or LC-MS/MS Capillary (Capellini™), Guard/Trap 0.3 - 1mmID (Piccolo™) or Direct Connect Ballistic Gradient, 2mmID (Sprite™) configurations.
HAISIL PS Phalanx Ultra-high density coating for wide pH range applications as columns or in LC-MS/MS Capillary (Capellini™), Guard/Trap 0.3 - 1mmID (Piccolo™) or Direct Connect Ballistic Gradient, 2mmID (Sprite™) configurations.

Amino acids on HAISIL Columns:
Targa (low organic, wettable RPC) for DABSYL or AccQ-Tag® derviatives
High Load for fast PTH analyses
HAISIL 100 for Pico-Tag® derivatives

Clipeus (WOW! selectivity) in C8, C18, CN or Phenyl phases that do not require base deactivation to chromatograph small polar amines without tailing; or for LC-MS/MS "no TFA" peptide separations.
Combi-Chem analyses of basic molecules on HAISIL HL "High Load" columns
Targa. for 10x faster equilibration using 100% aqueous conditions for an extremely wide polarity range of compounds; or for LC-MS/MS "no TFA" peptide separations.
Phalanx Ultra-high density coating for wide pH range applications. Also for 10x faster equilibration using 100% aqueous conditions.

Maccel™ Applications
Enhanced polar selectivity on MACCEL AQPS™, a water wettable (low organic RPC) phase, with great selectivity for all polar molecules.
Engelhard Test: Polar Selectivity of unbuffered acids and bases on AQPS phases.
Polar Selectivity of acids and bases on AQPS phases under acidic buffered, ACN conditions.
Optimum Conditions for acids and bases on AQPS phases: neutral buffered, MeOH conditions.

Compare selectivity on MACCEL Spheribond™ ODS1 and ODS2 to Waters™ Spherisorb® ODS1 and ODS2
Spheribond™ ODS1: Selectivity is similar under a number of comparative conditions.
Spheribond™ ODS2: Selectivity is very close under a number of comparative conditions.

Polar molecule selectivity on MACCEL Hypersorb™ ODS is very close to Thermo™ Hypersil® ODS
Hypersorb™ ODS: Selectivity under a number of comparative conditions is very similar.

Maccel™ Applications pdf
Alcohols 1
Aldehydes & Ketones
Amino Acids 1
Aminolevulinic Acid
Animal Food
Antirheumatic Bath Ingredient
Carotinoids 1
Castor Oil
Catecholamines
Cold Medicine
Dihydroxybenzoic Acid
Englehardt QC Test for RP Phases
Fat Soluble Vitamins 1
Fat Soluble Vitamins 2
Fatty Acid
Formic Acid / Acetic Acid
Hops 1, Bitter Ingrediants of
Hops 2, Bitter Ingrediants of
Isulin Derivatives 1
Isulin Derivatives 2
Iodate in Galvanic Bath
Isomers Vitamin E Tocopherol
Ketones
Melatonin
Organic Acids 1
Organic Acids 2
Organic Acids 3
Parabens
Peptides 1
Peptides 2
Peroxide
Phenol
Poly-Siloxane-Oil
Proteins 1
Proteins 2
Proteins 3
Soybean Oil
Sugars 1
Sugars 2
Tricyclic Antidepressants 1
Tricyclic Antidepressants 2
Ubiquinols
UV vs. ELSD
Water Soluble Vitamins
Weak Anions, Ion Pair Chrom.
Xanthines 1, N-Methylated

Vydac® Columns for Peptide (TP, MS, and Everest™) and Small Molecule (Denali™) Separations.
Application Note pdfs
Column Care
Cross Reference Guide
New! Everest™ C18 (monomeric) Ultra High Resolution Protein & Peptide Tryptic Mapping Columns. Comparison using formic acid to 100Å (LC Packings) column. "238EV™ Series".
Importance of TFA Concentration for a reproducible peptide separation.
Low TFA columns
Mobile Phase modifier effects on bonded phase selectivity
Monomeric C18 , 238TP RPC chemistry
Process scale TP and 259VHP medias for 50 to 150mmID prepacked columns.
Proteomic applications on low TFA MS media in prepacked capillary columns from 0.075mmID - 0.500mmID.
Validation of Reverse Phase HPLC separations (TFA, PO4, HOAc, HCl)
New! Denali™ C18 (monomeric) C18 provides excellent peak symmetry for basic and acidic compounds with superior efficiency and reproducibility for Small Molecules. Prices at "238DE™ Series" Prices.

Size Exclusion Chromatography (SEC) of Proteins

SEC Separations on HYDROXYETHYL Aspartamide columns pdf
Fast SEC of Drug-Protein Conjugates by SEC pdf

Process Columns

Adjustable, Empty Glass Process Columns. An overview of properties and capabilities.

For more information or to place an order contact:
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Last Updated: 07/13/07
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