Strong Cation Exchange (SCX) precolumn fractionation coupled directly to RPC-MS-MS simplifies complex protein digest characterization for increased productivity. Whether Multidimensional Chromatographic Analysis of Proteins (MudPIT), Signature Peptides, the DALPC method (Direct Analysis of Large Protein Complexes), ICAT™ (isotope-coded affinity tagged) labeled, or specific amino acid containing peptides are desired from digested proteins for proteomic characterization, this SCX column in tandem with a capillary RPC column allows true 2-D chromatographic fractionation of 1,500-2000 peptides per run at 5 orders of magnitude better detectability than 2-D gel electrophoresis.Use of the 200Å pore SCX packings will allow the isolation of +1 peptides (C-terminal, blocked N-terminal and phosphopeptides) in the first three peaks after injection, prior to starting the salt gradient for off-line 2-D fractionation. Use of the 4.6mmID x 50mm column affords >1mg capacity with the added benefit of allowing a greater number of injections before requiring the cleaning of trypsin from the column. The 2.1mmID x 50mm SCX column would allow > 0.2mg capacity. For information of these products see our complete PolyLC price list.
Last Updated: 08/13/09