UltraMicro™ Spin Columns are available in RPC solvent resistant housings for purification of small samples (3-30µg). UltraMicroSpin Columns offer a wide variety of packing materials including gel-filtration (no sample capacity, and they have sample volume limits), ion-exchange, silica based reverse and normal phase materials. Additionally, specific packing materials like HILIC for detergent removal, HIL-SCX for post ICAT sample prep, and TARGA C18 for (100% water elution) desalting of carbohydrates and heightened retention of polar molecules. Empty columns are available so your proprietary packings materials can be used in this format.

UltraMicro Spin Columns have a collar for microfuge centrifugation or they can be used with a syringe like the UltraMicroTip columns. Packing bed volume is ~50µL, with sample elution volumes of 2-25µL.

Use these columns with centrifugation, vacuum or pressure to solve the most demanding desalting problem for your protein, DNA, or carbohydrate. The recovery is reproducible and there is almost no sample loss.

PAGE*Eraser™ microFilter Spin Columns remove particulates from samples from in-gel digests to prolong capillary LC-MS column life.

MACROspin columns have a packing volume of 150-300µL, suitable for 50-150µL sample volumes for desalting by SEC (limit sample volume load to <100µL), or desalting by partition and elution with larger volumes.

96-Well Spin Plate are now formated as both MiniSpin and MacroSpin plates to bring speed and simplicity to the parallel processing of microSPE.

Micro Tip Columns for micro-pipettors are the easy to use UltraMicroSpin columns without the removable collar. Sample can be applied either by application at the top of the column or by suction through the column, using a micro-pipettor. Up to 20 different types of column materials are available. Care must be taken to limit the number of cycles of sample solvent loading, since elution of sample can occur when more than ten bed volumes are employed for loading and wash. Additionally, the rate of solvent loading and elution will affect the ability of one's sample to diffuse onto or off of the bed. For these reasons, we prefer the use of MiniSpin columns rather thanTips. However, there is the convenience factor that a Tip offers, which we cannot deny.

Detergent Removal: Use HILIC MiniSpin columns for complete detergent removal.

Volume Dependent Results for Salt Removal by SEC:

	Bed Volume	Loading Volume	Desalting Efficiency
96-Well MacroSpin Plate	125µL	125µL	100%
	125µL	250µL	>99%
	125µL	400µL	78%

Volume Dependent Results for Retention with Partition Column Chemistries:

Desorption increases with an excess of sample loading volume and/or wash volume. The maximum loading volume is one chromatographic void volume (ca. 60% of the bed volume) less than the k' of the analyte of interest. Increased loading can be obtained by either selecting a more retentive solvent condition or from a more retentive column chemistry such as TARGA C18, which allows loading in 100% water and which could even retain (for a few column volumes) underivatized simple sugars.

Effect of excessive loading and wash volumes on SPE Tips or Trap Columns
Data, courtesy of Brian Hampton, University of Maryland, generated from a trapping experiment on a 0.3 x 30mm, TARGA C18, capillary trap. Various volumes of sample in 0.1% formic acid loaded onto a trap column equilibrated with 0.1% formic acid.

Overview of MiniSpin Columns and Tips

Multiple Column Chemistries Provide Selective Separations:
(A) A mixture of BSA (MW 68,000) and Vitamin B-12 (MW 1355) shows two peaks in HPLC - Chromatograms after sample preparation with UltraMicro-Spin column (Sample Vol. 20µL).

(B) After centrifugation with G-50 (size exclusion) spin column, the smaller molecule (Vitamin B-12) is retained and BSA is eluted. This MiniSpin column can be used for the removal of small molecules such as salts or primers from oligonucleotides.

(C) After centrifugation with a C-18 (reverse phase) MiniSpin column, BSA is retained and Vitamin B-12 is eluted. This column chemistry can be used for the analysis of small molecules after the removal of proteins or large molecules as would occur in plasma samples.

(D) After centrifugation with a Silica (normal phase) MiniSpin column, BSA is eluted and Vitamin B-12 is retained. This column chemistry can be used for the analysis of small as well as large molecules. By using different eluents small molecules can be eluted from column after removal of large molecules.

APPLICATIONS:

Affinity chromatography	Peptides
Buffer exchange	Primer removal
Carbohydrates	Proteins
DNA	Radiolabel removal
Detergent removal	Removal of salt
Nick translation	Removal of small molecules
Removal of particles

See MiniSpin Columns and MicroTip Kits for Part Numbers and Prices.

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Copyright© 1995-2012 The Nest Group, Inc. All rights reserved (established 1984)

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For more information or to place an order contact:
The Nest Group, Inc.· 45 Valley Road · Southborough, MA  01772-1323
Tel: 800-347-6378 or 508-481-6223 · Fax: 508-485-5736
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Last Updated: 12/23/11