UltraMicro™ Spin Columns are available in RPC solvent resistant housings for purification of small samples (3-30µg). UltraMicro Spin Columns offer a wide variety of packing materials including gel-filtration (no sample capacity, and they have sample volume limits), ion-exchange, silica based reverse and normal phase materials, as well as specific packing materials like HILIC for detergent removal and SCX for post ICAT sample prep. Empty columns are available so your proprietary packings materials can be used in this format.
UltraMicro Spin Columns have a colar for microfuge centrifugation or they can be used with a syringe like the UltraMicroTip columns. Packing volume is 50µL, with sample elution volumes of 2-25µL.
Use these columns with centrifugation, vacuum or pressure to solve the most demanding desalting problem for your protein, DNA, or carbohydrate. The recovery is reproducible and there is almost no sample loss.
PAGE*Eraser™ microFilter Spin Columns remove particulates from samples from in-gel digests to prolong capillary LC-MS column life.
MacroSpin columns have a packing volume of 500µL, suitable for 50-400µL sample volumes for trace enrichment.
96-Well Spin Plate are now formated as both MiniSpin and MacroSpin plates to bring speed and simplicity to the parallel processing of microSPE.
Micro Tip Columns for micro-pipettors are easy to use UltraMicroSpin Columns. Sample can be applied either by application at the top of the column or by suction through the column, using the micro-pipettor. Up to 20 different types of column materials are available.
Detergent Removal: Use HILIC MiniSpin columns for complete detergent removal.
Volume Dependent Results for Salt Removal by SEC:
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APPLICATIONS:
Multiple Column Chemistries Provide Selective Separations
(B) After centrifugation with G-50 (size exclusion) spin column,
the smaller molecule (Vitamin B-12) is retained and BSA is eluted. This
MiniSpin column can be used for the removal of small molecules such as
salts or primers from oligonucleotides.
(C) After centrifugation with a C-18 (reverse phase) MiniSpin
column, BSA is retained and Vitamin B-12 is eluted. This column chemistry
can be used for the analysis of small molecules after the removal of proteins
or large molecules as would occur in plasma samples.
(D) After centrifugation with a Silica (normal phase) MiniSpin
column, BSA is eluted and Vitamin B-12 is retained. This column chemistry
can be used for the analysis of small as well as large molecules. By using
different eluents small molecules can be eluted from column after removal
of large molecules.
MICROTIP COLUMNS
Figure 1. See MiniSpin Columns and MicroTip Kits for Part Numbers and Prices.
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(A) A mixture of BSA (MW 68,000) and Vitamin B-12 (MW 1355) shows
two peaks in HPLC - Chromatograms after sample preparation with UltraMicro-Spin
column (Sample Vol. 20µL).
The Nest Group, Inc.· 45
Valley Road · Southborough, MA 01772-1323
Tel: 800-347-6378 or 508-481-6223 ·
Fax: 508-485-5736
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Last Updated: 02/10/06