The
Role of Column Length
in
Gradient Separations
Changes in column
length have a direct and linear effect on theoretical plates and analysis
time in isocratic separations. Column length plays a less important
role, however, in gradient techniques. The three chromatograms below
illustrate how well packed short HPLC columns yield very similar peptide
separations under identical conditions. For this study, the 2.1mm
I.D. HAISIL 300 C18
5µm columns ranged from 250 to 100mm in length. While
solvent and analysis time savings may be minimal, column cost savings can
be significant when using as short a column as possible.
1. Gly-Tyr
2. Val-Tyr-Val
3. Met Enkephalin
4. Leu Enkephalin
5. Angiotensin II
| HAISIL 100
| PROTO200
| PROTO300
| HAISIL 300
| HAISIL HL
| PHALANX
| HEAVY |
| HAISIL PTC
| HAISIL PTH
| PL Gel, PLRP-S
| TARGA
| CLIPEUS
|
| Higgins Index
| Kromasil Columns
| Haisil Cartridge Columns
| Haisil Col.Protection Prods
|
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