Complex Carbohydrate Analysis Columns
Complex Carbohydrate Analysis Columns
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PolyGLYCOPLEX™ for Complex Carbohydrate HPLC Using HILIC Conditions
The PolyGLYCOPLEX columns permit the separation of complex carbohydrates in volatile mobile phases; typically, acetonitrile and water, and due to their higher polarity surface, they require less acetonitrile than the PolyHYDROXYETHYL Aspartamide HILIC columns. Direct flow to Mass Spectrometry is convenient. Compared to reversed-phase HPLC, selectivity is generally superior. Selectivity is quite good for both native glycans and derivatives such as those with the 2-amino pyridine (PA-) fluorophore. The complete library of complex carbohydrates from a particular glycoprotein can often be resolved with isocratic elution, although gradients are recommended for especially varied samples.
Some advantages over basic HPLC materials (e.g., amino-silica) are:
1. Sialylated and asialo-glycans can be resolved using the same running conditions.
2. It is not necessary to operate at pH 7.3 or above with PA-derivatives or other reductively-aminated derivatives of carbohydrates.
For a discussion of the application of this chemistry, see Publications.
PolyGLYCOPLEX is available in analytical and preparative columns or as bulk media for capillary column packing.
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Last Updated: 02/21/08
