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PolyLC® Column Chemistries Bibliography

PolyCAT A® PolyAspartic Acid weak cation exchange publications
PolyHYDROXYETHYL® Aspartamide SEC or HILIC publications.
PolyALKYL® Aspartamide HIC publications.
PolySULFOETHYL® Aspartamide SCX strong cation exchange or HILIC publications.
Proteome fractionation by SCX and RPC-MS-MS.
PolyWAX LP® weak anion exchange publications.
PolyGLYCOPLEX for HPLC of Complex Carbohydrates.


PAPERS on PolyCAT A® and PolyAspartic Acid®


February 22, 2005	Weak Cation Exchange (WCX ) and HYDROPHILIC-INTERACTION CHROMATOGRAPHY (HILIC)
Ref#
SOURCE
SUBJECT
C1)
 Alpert, J. Chromatogr. 266 (1983) 23.
Description of PolyCAT A.
C2)
 Ou et al., J. Chromatogr. 266 (1983) 197.
Analysis of hemoglobin variants.
C3)
 Parente and Wetlaufer, J. Chromatogr. 288 (1984) 389.
Effect of urea on cation-exchg of lysozyme.
C4)
 Aswad, J. Biol. Chem. 259 (1984) 10714. 
Cation-ex. of ACTH.
C5)
 Parente and Wetlaufer, J. Chromatogr. 314 (1984) 337.
Effect of heat & urea on cation-exchg. of chymotrypsinogen
C6)
 Ellis et al., Clin. Chem. 30 (1984) 1746.
Assay of Hemoglobin A1c.
C7)
 Rogers et al., Am. J. Clin. Pathol. 84 (1985) 671.
Analysis of hemoglobinopathies.
C8)
 Ou and Carmona, Clin. Chem. 31 (1985) 945.
Assay of Hemoglobin A1c (abstract only).
C9)
 Parente and Wetlaufer, J. Chromatogr. 355 (1986) 29.
Isocratic vs. gradient elution of proteins.
C10)
Wessels et al., Clin. Chem. 32 (1986) 903.
Diagnosis of Hemoglobin S/Lepore.
C11)
Turpeinen, Clin. Chem. 32 (1986) 999.
Assay of Hemoglobin A2.
C12)
Langer et al., Anal. Biochem. 166 (1987) 212.
Purif. of N-Glycosidase F.
C13)
Spiker et al., J. Chromatogr. 405 (1987) 327.
Fractionation of HMG proteins.
C14)
Bisse and Wieland, J. Chromatogr. 434 (1988) 95.
Assay of Hemoglobins F and A1c.
C15)
Kavanaugh et al., J. Biol. Chem. 264 (1989) 11009.
Purif of ATP-labeled hemoglobin.
C16)
Marchese et al., J. Chromatogr. 504 (1990) 351. 
Separation of three forms of rh-Interleukin-2.
C17)
Canova-Davis et al., J. Chromatogr. 508 (1990) 81.
Characterization of synthetic human relaxin.
C18)
Ebert and Spryn, Bioconjugate Chem. 1 (1990) 331.
Purif. of a Ribosome-Inactivating Protein from barley.
C19)
Osheroff et al., J. Biol. Chem. 265 (1990) 9396.
 Prepn. of 32P-labeled human relaxin.
C20)
Kutlar et al., Hum. Genet. 85 (1990) 106.
Assay and purif. of Hemoglobin F.
C21)
Rao et al., BioPharm 4 (#10) (1991) 38. 
Separation of post-translational variants of monoclonal antibodies.
C22)
Huisman, Sangre 36 (1991) 144.
HPLC of abnormal hemoglobins.
C23)
Lee et al., Diabetes Res. Clin. Pract. 16 (1992) 53.
HPLC of glycated proteins vs. home blood glucose testing.
C24)
Holmes et al., Science 256 (1992) 1205. 
Isoln. of heregulin (an oncogene product activator).
C25)
Burton et al., J. Neurochem. 59 (1992) 1937.
Analysis of various forms of rNerve Growth Factor.
C26)
Fu et al., J. Biol. Chem. 266 (1991) 14562.
Affinity-style isoln. of an L-Isoaspartyl Protein Carboxyl Methyltransferase.
C27)
van der Dijs et al., Clin. Chem. 38 (1992) 1864.
Screening cord blood for hemoglobinopathies and thalassemia.
C28)
Ou and Rognerud, Clin. Chem. 39 (1993) 820.
Rapid screening for hemoglobin variants.
C29)
Kutlar and Huisman, J. Chromatogr. 620 (1993) 183.
Micro HPLC method for determining the g chain composition of hemoglobin F.
C30)
Hazelett et al., Alcohol. Clin. Exp. Res. 17 (1993) 1107.
Screening Hemoglobin-acetaldehyde as a marker for alcoholism.
C31)
Gibbs et al., Proc. Natl. Acad. Sci. 88 (1991) 4001.
Purif. of single-chain catalytic antibody.
C32)
Pantoliano et al., Biochemistry 30 (1991) 10117.
Purif. of single-chain anti-fluorescein antibody
C33)
Milenic et al., Cancer Res. 51 (1991) 6363.
Purif. of single-chain anti-carcinoma antibody.
C34)
Stewart et al., Biochemistry 33 (1994) 1994.
Purif. of catalytic antibody.
C35)
Richard et al., Amer. Clin. Lab. (1/1994) 26.
Assay of Hemoglobin A1c.
C36)
Lu et al., Chromosoma 103 (1994) 111.
Separation of Histone H1 phosphorylation variants from Tetrahymena.
C37)
Itala et al., Anal. Biochem. 224 (1995) 323.
Hemoglobin-acetaldehyde analysis.
C38)
Fiordalisi et al., Protein Exp. Purif. 3 (1992) 282 
Purif. of recombinant k-Bungarotoxin.
C39)
Pearson et al., Hemoglobin 18 (1994) 401.
HPLC of rat hemoglobins.
C40)
Thomas et al., Science 241 (1988) 226.
Analysis of prohormone versions of (-endorphin and (-lipotropin.
C41)
Thorne et al., J. Biol. Chem. 264 (1989) 3545.  
Further study of prohormone processing.
C42)
Thomas et al., J. Biol. Chem. 265 (1990) 10821. 
More prohormone processing.
C43)
Thomas et al., Proc. Natl. Acad. Sci. 88 (1991) 5297.
Still more prohormone processing.
C44)
Pic et al., Ann. Biol. Clin. 52 (1994) 129.
Assay of Hemoglobin F, Fac, & variants..
C45)
Jones, Meth. Enzymol. 231 (1994) 322.
Chemically-modified hemoglobins.
C46)
Turpeinen et al., Clin. Chem. 41 (1995) 191.
Assay of Hemoglobin A1c; comparison of PolyCAT A with Diamat and affinity methods.
C47)
Turpeinen et al., Clin. Chem. 41 (1995) 532.
Assay of Hemoglobin (-chain variants: Broussais & Cemenelum.
C48)
BissÈ et al., Clin. Chem. 41 (1995) 658.
Elevation of Hemoglobin A1d in uremia.
C49)
Cuevas et al., Clin. Chem. 41 (1995) S98 (abstract 292) 
Retention of Hemoglobin variants relative to the Hb A peak.
C50)
Ou & Rognerud, ibid, S100 (abstract 300).
Assay of Hemoglobins Chicago & South Florida
C51)
Ou & Rognerud, Clin. Chem. 42 (1996) 774.
Assay of Hemoglobin Chicago/S trait and Hb Chicago/Sickle Cell disease.
C52)
Shahrokh et al., Pharm. Res. 11 (1994) 936.
 Basic Fibroblast Growth Factor: Isoln. of an Asp15 == succinimide 15 variant and misc. cleavage variants.
C53)
BissÈ et al., J. Chromatogr. B 687 (1996) 349.
Elevation of Hemoglobins A1d3a and A1d3b in uremia and diabetes, resp.
C54)
Vunnam et al., J. Peptide Res. 49 (1997) 59.
Purif. of synthetic cecropin and Pro-Arg-  rich peptides from pig intestine.
C55)
Chen et al., Alcohol Clin. Exp. Res. 19  (1995) 939.
New Hemoglobin-acetaldehyde variants.
C56)
Wilson et al., J. Lab. Clin. Med. 108 (1986) 138.
Analysis of hemoglobins S, C, and O Arab in cord blood samples.
C57)
Fishman et al., J. Chromatogr. 532 (1990) 55.
Separation of pyridoxylated hemoglobins.
C58)
Galacteros et al., Blood 78 (1991) 1121.
Adult hemoglobin conc. vs. gestational age in newborns.
C59)
Roa et al., Ann. Clin. Lab. Sci. 23 (1993) 433. 
Hemoglobin variant detection in dried blood specimens.
C60)
Roa et al., Ann. Clin. Lab. Sci. 25 (1995) 228. 
Reference ranges for hemoglobin variants in African Americans.
C61)
Garibay-Tupas et al., J. Endocrinol. 145 (1995) 441.
Prepn. of 32P-labeled human relaxin.
C62)
Huber et al., Prenat. Diagn. 16 (1996) 1011.
Estimating hemoglobin F from fetal cells isolated from maternal blood.
C63)
Leonova et al., Am. J. Hematol. 53 (1996) 59.
Variability in hemoglobin F level of normal adults.
C64)
John et al., Clin. Chem. 43 (1997) 968. 
Evaluation of a hemoglobin analyzer; PolyCAT A as the gold standard
C65)
Lindner et al., J.Chromatogr. A. 782 (1997) 55.
Purif. of phosphorylated histones by hydrophilic interaction chromatography.
C66)
McDonald et al., Protein Exp. Purif 8 (1996) 97.
QC analysis for deamidated variant of a recombinant fusion protein of basic fibroblast growth factor-saporin mitotoxin.
C67)
Frantzen, J. Chromatogr. B 699 (1997) 269.
Modified hemoglobins: A review of analytical methods.
C68)
Mario et al., Clin. Chem. 43 (1997) 2137.
Comparison of HPLC and IEF forhemoglobin variant analysis.
C69)
Pearson et al., Hemoglobin 22 (1998) 245. 
Reversing the Hb F to Hb A switch in rats.
C70)
Hazelett et al., Alcohol Clin. Exp. Res. 22 (1998) 1813.
Comparison of Hemoglobin-acetaldehyde with CDT and other alcoholism markers.
C71)
Sanchez et al., Thrombosis Res. 87 (1997) 289.
Purif. of chimeric fibrolase attached to an RGD-like peptide.
C72)
Lindner et al., J. Cancer Res. Clin. Oncol. 125 (1999) 182.
Deamidation and N-terminal modification of H1 histones as a function of age.
C73)
Levandoski et al., J. Neurochem. 74(3) (2000) 1279.
Recombinant Expression of alpha-Bungarotoxin. Use of 40% organic solvent in mobile phase improved resolution of toxin separations.
C74)
Parsell et al., J. Biol. Chem. 271 (1996) 27936.
Separation of 32P-phosphorylated from native.relaxin.
C75)
Sweet et al., J. Biol. Chem. 272 (1997) 916.
Isoln. of phosphorylated Lys-C fragments of linker histones from Tetrahymena.
C76)
Al-Abed et al., Proc. Natl. Acad. Sci. 96 (1999) 2385.
Separation of rat hemoglobins with adducts of.glucose, acetaldehyde, and advanced glycation endproducts.
C77)
Paleari et al., Clin. Chem. 45 (1999) 21.
HPLC of Hemoglobin J Sardegna, with a post- translational deamidation.
C78)
Campbell  et al., Cytometry 35 (1999) 242.
HPLC of Hemoglobin F to correlate results from flow  cytometry of erythrocytes.
C79)
Bohmer et al., Blood 95 (2000) 2967.
Stimulation of Hb F production by TGF-b; Hbs F and A measured by HPLC and flow cytometry.
C80)
Roberts et al., Clin. Chem. 46 (2000) 569.
Glycohemoglobin measurements with Hb variants.
C81)
Bissé et al., J. Biol. Chem. 275 (2000) 21380.
Detection of a Thr® Ala replacement in Hb Saale.
C82)
Bates et al., J. Biol. Chem. 275 (2000) 6234.
Purif. of a Fe-S cluster transcription factor protein, FNR, from E. coli.
C83)
Theodorakis et al.,, Biochim. Biophys. Acta 1252 (1995) 114.
Cytochrome C derivs. with thiopropyl- groups at lys-;sepn. of positional variants..
C84)
Theriault et al., Nucl. Acids Res. 13 (1985) 8441
Purif. of PaeR7 endonuclease & methylase.
C85)
Xu et al., J. Biol. Chem. 275 (2000) 17086..
Purif. of restriction endonuclease Hpy188I from Helicobacter pylori.
C86)
Zhang et al., J. Chromatogr. B. 759 (2001) 1.
Characterization of glycated hemoglobin in diabetics using CEX and ESI-MS.
C87)
Nakanishi et al., Clin. Chem. 46 (2000) 1689.
Chromatography vs. ESI-MS: Effect of hemoglobin variants on measurement of glycation.
C88)
Balhorn et al., Environ. Health Perspect. 62 (1985) 73.
Isoln. of albumin and hemoglobin from blood for measurement of mutagen adducts.
C89)
 Wimley and White, Biochemistry 39 (2000) 4432.
Designer transmembrane peptide purified with an acetic acid gradient.
C90)
Ashish and Wimley, Biochemistry 40 (2001) 13753.
Acetic acid gradient used to purify peptides that interact with membrane proteins.
C91)
Ou and Rognerud, JClin. Chim. Acta 313 (2001) 187.
Diagnosis of hemoglobinopathies:  HPLC is better than electrophoresis.
C92)
Lackmann et al., J. Biol. Chem. 267 (1992) 7499.
Purif. of a murine chemotactic cytokine.
C93)
Kiseleva et al., Analyst 126 (2001) 2119.
Ion chromatography: Simultaneous analysis of anions and cations on a PolyCAT A column.
C94)
Wojda et al., Blood 99 (2002) 3005.
Study of Hb F and A production in vitro by differentiating erythroid cells.
C95)
Ghosh et al., Nature Immunol. 3 (2002) 583.5.
Acetic acid gradient for purification of defensin HD-from Paneth cells.
C96)
Nakahishi et al., Clin. Chim. Acta 323 (2002) 89-101.
Assessment of hemoglobin variantsÕ effect on routine Hb A1c measurements; CEX and ESI-MS.
C97)
Lamberty et al., J. Biol. Chem. 274 (1999) 9320.
Purif. of a recombinant defensin from tobacco budworm.
C98)
Al-Abed et al., Mol. Med. 7 (2001) 619.
Measurement of Hemoglobin A1d3, a glutathione adduct form elevated in diabetics.
C99)
Cokic et al., J. Clin. Invest. 111 (2003) 231.
Induction of Hemoglobin F by hydroxyurea via NO activation of guanylyl cyclase.
C100)
Kang & Frey, J. Chromatogr. A, 991 (2003) 117.
Chromatofocusing of proteins.
C101)
Ho et al., Exp. Hematol. 31 (2003) 586.
Induction of Hemoglobin F with hydroxyurea by inhibition of ribonucleotide reductase.
C102)
Bhanu et al., Blood (online ed. 10/30/03).
Signaling factors that increase Hemoglobin F levels.
C103)
Mohanty et al., J. Biol. Chem. 278 (2003) 40953.
Removal of DTT & other contaminants from synth. e-colicin peptide with a PolyCAT A SPE cartridge with elution with acetic acid.
C104)
Wittamer et al., J. Exp. Med. 198 (2003) 977.
Purif. of chemerin, a chemoattractant protein, via RPC-WCX-RPC.
C105)
Bathgate et al., Biol. Reprod. 67 (2002) 293.
Isoln. of wallaby relaxin.
C106)
Uzzell et al., Peptides 24 (2003) 1655. 
Hagfish antimicrobial peptides; conversion of TFA to acetate salts. 
C107)
Howell et al., Peptides 24 (2003) 1763. 
Antimicrobial peptides from colon; isoln. with acetic acid gradient. 
C108)
BissŽ et al., Clin. Chem. 49 (2003) 137. 
Isoln. of Hemoglobin Gšrwihl. 
C109)
Levasseur et al., J. Biol. Chem. 279 (2004) 27518. 
Purif. of a recombinant anti-sickling hemoglobin. 
C110)
Harris et al., J. Biol. Chem. 279 (2004) 20250. 
Isoln. (acetic acid gradient) of antimicrobial peptide from chinchilla airway. 
C111)
Sutton et al., J. Bacteriol. 186 (2004) 8018. 
Isoln. of FNR protein (see also ref. C82). 
C112)
Claasz et al., Eur. J. Biochem. 269 (2002) 6287. 
Purity analysis of relaxin analogs. 
C113)
Toke et al., Biophys. J. 87 (2004) 662. 
Peptide antibiotic converted from TFA to acetate form. 
C114)
Bhanu et al., Blood 105 (2005) 387. 
Enhanced production of Hemoglobin F in adult erythroid precursor cells monitored with PolyCAT A. 
C115)
Amano et al., Infect. Immun. 66 (1998) 2072. 
Purif. of proline-rich glycoproteins from saliva. 
C116)
 Morgan et al., Appl. Env. Microbiol. 64 (1998) 3669. 
Isoln. of the thermostable restriction endonuclease PspGI. 

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PAPERS on PolySULFOETHYL Aspartamide®

February 22, 2005	Strong Cation Exchange (SCX) and HYDROPHILIC-INTERACTION CHROMATOGRAPHY (HILIC)
Ref#
SOURCE
SUBJECT
S1)
Alpert and Andrews, J. Chromatogr. 443 (1988) 85.
Description of PolySULFOETHYL A and SCX of peptides.
S2)
Crimmins et al., J. Chromatogr. 443 (1988) 63.
SCX of peptides.
S3)
Andrews, Peptide Res. 1 (1988) 93.
Ion-exchange HPLC of peptides.
S4)
Crimmins et al., Biochem. Biophys. Res. Commun. 156 (1988) 910.
SCX analysis of deblocking of N-terminal pyroGlu- peptides.
S5)
Crimmins et al., Anal. Biochem. 176 (1989) 255.
Peptide mapping of V8 digests by SCX.
S6)
Schlabach et al., Techniques in Protein Chem. 1 
(Academic Press; 1989), 497.
Peptide mapping of ASP-N digest.
S7)
Crimmins, Peptide Res. 2 (1989) 395.
SCX of disulfide-linked peptides.
S8)
Kolbe et al., J. Chromatogr. 476 (1989) 99.
Purif. of recombinant p18 protein from HIV-1 virus.
S9)
Andrews, Current Res. Protein Chem. (Academic Press; 1990) 95.
Selective isolation of disulfide-linked peptides from tryptic digests.
S10)
Iadarola et al., J. Chromatogr. 512 (1990) 165.
Albumin variant digests by CNBr, trypsin, and V8; SCX vs. RPC.
S11)
Sandy et al., J. Biol. Chem. 265 (1990) 21108.
Isoln. of disulfide-linked peptides from aggregating cartilege proteoglycan.
S12)
Guarino and Phillips, Amer. Lab. 23 (1991) 68.
SCX-HPLC of peptides as a preparative adjunct to CZE.
S13)
Swiderek et al., Eur. J. Biochem. 190 (1990) 575.
V8 digest of Troponin T;  mapping sites of phosphorylation.
S14)
Alpert, HPLC of Peptides and Proteins (Mant & Hodges, eds.) 
(CRC Press; 1991) 187.
IEX of peptides.
S15)
Crimmins, Techniques in Protein Chem. III 
(Academic Press; 1992) 171.
SCX vs. CZE of synthetic peptides.
S16)
Zhu et al., J. Chromatogr. 548 (1991) 13.
HILIC of peptides (same ref. as H2)
S17)
Zhu et al., J. Chromatogr. 594 (1992) 75.
SCX-HILIC of peptides vs. RPC.
S18)
Przysiecki et al., Protein Expression Purif. 3 (1992) 185.
Purif. of recombinant antistasin with a preproleader sequence (same ref. as H6).
S19)
Clogston et al., Anal. Biochem. 202 (1992) 375. 
Analysis of rG-CSF isoforms.
S20)
Schmeltzer et al., J. Neurochem. 59 (1992) 1675.
Isoln. & anal. of rh-Nerve Growth Factor.
S21)
Hempel et al., Comp. Biochem. Physiol. 102B (1992) 791.
SCX of tryptic digest of human liver glutamic g-Semi-al dehyde Dehydrogenas.
S22)
Churchill et al., Biochemistry 31 (1992) 3793.
SCX of tryptic digest of rat liver D-b-hydroxybutyrate dehydrogenase.
S23)
Moore et al., Proc. Natl. Acad. Sci. 90 (1993) 1354.
Isoln. of squalamine (a steroidal antibiotic).
S24)
Kieliszewski et al., Plant Physiol. 98 (1992) 919.
Purif. of hydroxyproline-rich glycoprotein from Douglas fir.
S25)
Kieliszewski et al., Plant Physiol. 99 (1992) 538.
Purif. of His- rich, arabinogalactan protein from corn (maize).
S26)
Fong et al., Plant Physiol. 99 (1992) 548.
Purif. of gymnosperm protein with serine-tetrahydroxyproline motif.
S27)
Longo et al., Cell Regulation 1 (1990) 189.
Purif. of synthetic peptide fragments of Nerve Growth Factor which inhibit NGF.
S28)
Hisatsune et al., Biosci. Biotech. Biochem. 56 (1992) 1616.
Purif. of a CNBr digest of casein.
S29)
Nichols, Molec. Cell. Neurosci. 3 (1992) 342.
Isolation of Drosophila Drosulfakinin Neural Peptide gene product, DSK-1.
S30)
Nichols, J. Mol. Neurosci. 3 (1992) 213.
Isolation of Drosophila neural peptides.
S31)
Tanasijevic et al., J. Biol. Chem. 268 (1993)18157.
Isolation  of phosphorylated fragments of Insulin Receptor Substrate (IRS-1).
S32)
Dolmer and Sottrup-Jensen, FEBS Letters 315 (1993) 85.
Isoln. of disulfide-linked peptides from human complement component C3b.
S33)
Gorman and Shiell, J. Chromatogr. 646 (1993) 193.
Selective isoln. of C-terminal and blocked N-terminal peptides from viral proteins.
S34)
Dong et al., J. Biol. Chem. 269 (1994) 6753.
Determination of disulfide bonds in von Willebrand Factor multimers.
S35)
Walker, Poster 272-T, Protein Society Symp. (7/94).
Assay of Growth Hormone-Releasing Factor by mixed SCX-HILIC.
S36)
Crimmins, Meth. Mol. Biol. 36 (1994) 53.
SCX in synthetic peptide analysis.
S37)
Hempel et al., Protein Sci. 3 (1994) 1074.
Purif. of peptides for sequencing from digest of UDP-glucose dehydrogenase.
S38)
Crimmins et al., Protein Sci. 3 (1994) 1074.
Disulfide-linked peptides; SCX & MALDI-MS.
S39)
Fang et al., J. Chromatogr. A 729 (1996) 49.
Effect of temperature on protein retention times in cation-exchange.
S40)
Fang et al., J. Chromatogr. A 729 (1996) 67.
Effect of temperature on protein bandwidth in cation-exchange.
S41)
Smith and Hanly, in Irvine and Williams (eds.), Neuropeptide Protocols 
(Methods in Molec. Biol. vol. 73), Humana Press Inc.,
Totowa, NJ, 1996, pg. 75.
a) Protocol for SCX of peptides; b) Purif. of crude synthetic 
Calcitonin Gene-Related Peptide (8-37).
S42)
Joyal et al., Biochemistry 35 (1996) 6267.
Identification of phosphorylation sites in calmodulin.
S43)
Bendixen et al., J. Biol. Chem. 270 (1995) 17929.
Isoln. of plasminogen fragments covalently linked ((-Glu Æ (-Lys) by transglutaminase treatment.
S44)
Mant & Hodges, Meth. Enzymol. 271 (1996) 3.
SCX of peptides: Hydrophilic & hydrophobic mixed-mode effects.
S45)
 Yarus et al., Proc. Natl. Acad. Sci. 93 (1996) 14118.
Purif. of bovine tracheal antimicrobial peptide (a ß-defensin) from transgenic mouse milk.
S46)
Cole et al., J. Biol. Chem. 272 (1997) 12008.
Isoln. of pleurocidin, an antimicrobial peptide from flounder skin.
S47)
Crimmins, Anal. Chem. Acta 352 (1997) 21.
SCX-HPLC as a tool for characterization and purif. of peptides.
S48)
Link et al., Nature Biotechnol. 17 (1999) 676.
Proteome analysis: SCX-RPC capillaries for direct MS/MS analysis of protein complexes.
S49)
Tseng et al., J. Biol. Chem. 271 (1996) 23992.
Purif. of human type II phospholipase A2 from  CHO cells (SCX-RPC sequence).
S50)
John et al., Proc. Natl. Acad. Sci. 94 (1997) 10178.
Isoln. via SEC-SCX-RPC of a 17-residue peptide mediating glycolipid-induced tobacco 
protoplast proliferation in vitro.
S51)
Louie and Meade, Proc. Natl. Acad. Sci. 95 (1998).
Purif. of cobalt complexes of zinc finger peptides.
S52)
Giblin et al., Proc. Natl. Acad. Sci. 95 (1998) 12814.
Purif. of complex of rhenium with a-MSH.
S53)
Hekman et al., Pharm. Res. 15 (1998) 650.
Isoln. and ident. of degradation products of pramlintide (an amylin analog).
S54)
Hekman et al., J. Pharm. Biomed. Anal. 20 (1999).
Isoln. and ident. of cyclic imide and 763.deamidation products of pramlintide.
S55)
Ayad et al., J. Dental Res. 79 (2000) 976.
Isoln. of 19 basic, proline-rich peptides from human saliva; relationship to dental decay.
S56)
Gygi et al., Proc. Natl. Acad. Sci. 97 (2000)
Proteomics tools: Analysis of 2-D gel spots of yeast proteins using a trypsin SCX RPC MS/MS sequence.
S57)
Davis et al., Poster 196, 48th ASMS Conference (June 2000)
Proteomics analyses using a SCX-RPC-MS/MS setup.
S58)
Gygi et al., Nat. Biotechnol. 17 (1999) 994.
Introduction of ICAT™ (Isotope-Coded Affinity Tags) for analysis of complex protein mixtures.
S59)
Gygi et al., Curr. Opinion Biotechnol. 11 (2000) 396.
Review of ICAT™ and proteomics.
S60)
Blitvich et al., Virus Res. 60 (1999) 67.
Selective isoln. of C-terminal tryptic peptides from flavivirus NS1 protein.
S61)
Goodrum et al., Phytochem. 54 (2000) 99.
Purif. of chymotryptic peptides of gum arabic glycoprotein via a SCX-RPC sequence.
S62)
Yoshida et al., Brain Res. 894 (2001) 46.
Purif. of recomb. midkine neurotrophic factor.
S63)
Pearce et al., Proc. Natl. Acad. Sci. USA 98 (2001) 12843.
Isoln. from tobacco of RALF, a polypeptide hormone that arrests root growth & devel.
S64)
Verma et al., Mol. Biol. Cell 11 (2000) 3425.
Proteomics: SCX-RPC for identification of proteasomal proteins.
S65)
Davis et al., J. Chromatogr. B, 752 (2001) 281.
Proteomics: Assessment of SCX-RPC-MS/MS for proteomics analyses.
S66)
Griffin et al., Anal. Chem. 73 (2001) 978.
Proteomics: Identification of ICAT-labelled yeast proteins via ICAT labelling and SCX (offline)-RPC-MALDI QTOF MS.
S67)
Ideker et al., Science 292 (2001) 929.
Proteomics: Comparison of yeast protein and mRNA content changes (ICAT used).
S68)
Koc et al., J. Biol. Chem. 276 (2001) 43958.
Proteomics identification of proteins in the large subunit of bovine mitochondrial ribosome (ICAT used). 
S69)
Peng and Gygi, J. Mass Spectrom. 36 (2001) 1083.
Proteomics: A tutorial.
S70)
Gygi et al., J. Proteome Res. 1 (2002) 47.
Proteomics of low-abundance proteins: Assessment of reproducibility and sensitivity using SCX-RPC-MS/MS and ICAT.
S71)
Demond et al., AAPS PharmSciTech 1 (2000) article 6 (online journal).
Analysis of pramlintide by SCX and RPC.
S72)
Fayad et al., Appl. Microbiol. Biotechnol. 57 (2001) 117.
Purif. of a b-1,6-glucanase from Streptomyces sp. EF-14.
S73)
Lin et al., Amer. Genomic/Proteomic Technol. 1 (2001) 38.
MuDPIT and other approaches to proteomics of complex mixtures.
S74)
Han et al., Nat. Biotechnol. 19 (2001) 946.
Proteomics of microsomal proteins via ICAT.
S75)
Lee et al., Anal. Chem. 74 (2002) 4353.
Multiplexed capillaries for fast proteomics throughput.
S76)
Adkins et al., Mol. Cell Proteomics _1 (2002) 947.
Proteomics of human plasma; identification of 490 proteins.
S77)
Litowski and Hodges, J. Biol. Chem. 277 (2002) 37272. 
Effect of residue substitution on structure of heterodimeric two-stranded a-helical coils.
S78)
Ostrowski et al., Mol. Cell Proteomics 1 (2002) 451.
Proteomic analysis of human cilia.
S79)
Arnott et al., Mol. Cell Proteomics 1 (2002) 148.
Selective ICAT labeling and detection of membrane proteins without antibodies.
S80)
Jakubowski, J. Biol. Chem. 277 (2002) 30425.
Isoln. of homocysteine thiolactone from acid hydrolyzates of human plasma proteins.
S81)
Yvon et al., FEBS Lett. 247 (1989) 273.
Isoln. of tryptic fragments of HSA with benzyll penicilloyl- groups attached.
S82)
Raybould & Reeve, Peptides 17 (1996) 1307.
RPC-SCX purif. of CCK-58. 
S83)
Blitvich et al., J. Gen. Virol. 82 (2001) 2251.
Isoln. of the disulfide-linked tryptic fragment from NS1 protein from Murray Valley encephalitis virus via RPC-SCX. 
S84)
Baek et al., Circulation 105 (2002) 2779.
Use of a PolySULFOETHYL A cartridge to clean up a radioactive lys-lys derivative. 
S85)
Ršhrig et al., Proc. Natl. Acad. Sci. USA 99 (2002) 1915
Purif. of two peptides from soybean that regulate sucrose synthase. 
S86)
Overgaard et al., J. Biol. Chem. 278 (2003) 2106.
Selective isoln. of disulfide-linked peptides from CNBr-tryptic digest of the complex of 
pregnancy-associated plasma protein-A and eosinophil major basic protein.
S87)
Griffin et al., Mol. Cell. Proteomics 1 (2002) 323.
Proteomics of yeast Š number of proteins identified and quantified: A) RPC only: 34. 
B) SCX-RPC: 245. \SCX-RPC is better.
S88)
Adkins et al., Mol. Cell. Proteomics 1 (2002) 947.
Proteomics of human serum: 490 proteins identified via SCX-RPC-MS. 
S89)
Peng et al., J. Proteome Res. 2 (2003) 43.
Proteomics of yeast via SCX-RPC: ID of 7537 peptides from 1504 proteins. 
S90)
Froehlich et al., J. Proteome Res. 2 (2003) 413.
Proteomics of Arabidopsis thaliana chloroplast membranes: ID of 392 proteins viaoffline SCX-RPC-MS. 
S91)
Guina et al., J. Am. Soc. Mass Spectrom. 14 (2003) 742
Proteomics via ICAT of P. aeruginosa grown under magnesium limitation: ID of 1331 proteins, 
145 related to Mg+2 limitation.
S92)
Le Bihan et al., J. Am. Soc. Mass Spectrom. 14  (2003) 719.
Systematic exploration of variables in the SCX step of SCX-RPC-MS for peptides. 
S93)
Peng et al., Nat. Biotechnol. 21 (2003) 921.
Proteomics of ubiquitination in yeast: ID of 1,075 proteins with 110 ubiquitination sites. 
S94)
Tirumalai et al., Mol. Cell Proteomics 2 (2003) 1096.
Proteomics of the low mol. wt. components of serum: > 340 proteins identified. 
S95)
Vollmer et al., J. Biomol. Tech. 14 (2003) 128.
Proteomics of E. coli grown on different carbon sources. 
S96)
Cutillas et al., Clin. Sci. 104 (2003) 483.
ID via SCX-RPC-MS of > 100 peptides in desalted urine samples. 
S97)
Jakubowski & Guranowski, J. Biol. Chem. 278 (2003) 6765
Analysis of homocysteine thiolactone from plant extracts. 
S98)
Da Cruz et al., J. Biol. Chem. 278 (2003) 41566.
Proteomics of mouse liver mitochondrial inner membrane; ID of 182 proteins. 
S99)
Iijima et al., Eur. J. Biochem. 270 (2003) 675.
Purif. of three isoforms of chrysophsin, an antimicrobial peptide from gills of the red sea bream fish. 
S100)
Pearce & Ryan, J. Biol. Chem. 278 (2003) 30044.
Purif. of wound signal peptides from tomato leaves via RPC-RPC-SCX-RPC-RPC. 
S101)
Argirov et al., Biochim. Biophys. Acta 1620 (2003) 235.
Isoln. from calf lens protein of a pyridinium deriv. of lysine (an advanced glycation endproduct). 
S102)
Argirov et al., J. Biol. Chem. Nov. 21, 2003 (online).
Same pyridinium deriv. of lysine as S101. 
S103)
x…sapay et al., J. Biol. Chem. 275 (2000) 12017.
Isoln. of indolicidin with a Trp-Trp crosslink. 
S104)
Corbin et al., Proc. Natl. Acad. Sci. USA 100 (2003) 9232.
Proteome vs. the transcriptome of E. coli; 1,147 proteins identified. 
S105)
Barroso et al., J. Proteome Res. 2 (2003) 633.
Evaluation of a capillary RPC monolith in the SCX-RPC sequence for proteomics. 
S106)
Schmidt et al., Mol. Cell. Proteomics 3 (2004) 24.
M. tuberculosis proteome; 2-D electrophoresis vs. ICAT with SCX-RPC. 
S107)
Qiu et al., Anal. Chem. 74 (2002) 4969.
Simultaneous isoln. and labelling of Cys-containing peptides from a tryptic digest via covalent 
chromatography with subsequent SCX-RPC fractionation.
S108)
Wiedow et al., J. Biol. Chem. 265 (1990) 14791. 
Isoln. of elafin, an elastase inhibitor.  
S109)
Feurle et al., J. Biol. Chem. 267 (1992) 22305. 
Isoln. of xenin from gastric mucosa & effect on pancreas.  
S110)
Heine et al., J. Chromatogr. A, 776 (1997) 117. 
Analysis of urinary peptides.  
S111)
Tarver et al., Infect. Immun. 66 (1998) 1045. 
Purif. of enteric b-defensin.  
S112)
Sutton et al., Mol. Pharmacol. 54 (1998) 407. 
Purif. of peptide spider toxin DW13.3.  
S113)
Saboori et al., J. Immunol. 163 (1999) 6244. 
Separation of tryptic fragments of thyroglobulin.  
S114)
Needham et al., J. Chromatogr. B, 796 (2003) 347. 
Analysis of pyridostigmine in plasma via SCX/HILIC-ESI-MS.  
S115)
Ovsyannikova et al., Virology 312 (2003) 495. 
SCX-RPC isoln. of measles virus peptides from blood.  
S116)
Blonder et al., J. Invest. Dermatol. 123 (2004) 691. 
Proteomics of plasma membrane of human epidermis.  
S117)
Coulombe et al., Mol. Cell. Biol. 24 (2004) 6140. 
2-D LC detection of N-terminally ubiquitinated peptides from ERK3.  
S118)
Chan et al., Clin. Proteomics J. 1 (2004) 101. 
Analysis of human serum proteome via 2-D LC.  
S119)
Anderson et al., Mol. Cell. Prot. 3 (2004) 311. 
The human plasma proteome (a nonredundant list)  
S120)
MacKay et al., Mol. Cell. Prot. 3 (2004) 478. 
The transcriptome and quantitative proteomics of yeast response to mating pheromone. 
S121)
Lin et al., Mol. Cell. Prot. 3 (2004) 820. 
Quantitative proteomic & transcriptional response of follicular lymphoma cells.  
S122)
Yu et al., Mol. Cell. Prot. 3 (2004) 896. 
Proteomics of cortical neuron.  
S123)
Tian et al., Mol. Cell. Prot. 3 (2004) 960. 
Incongruent expression of transcription and proteomic response in different cells.  
S124)
Ross et al., Mol. Cell. Prot. 3 (2004) 1154. 
Introduction of iTRAQ˚: Proteomics of yeast.  
S125)
Shen et al., Anal. Chem. 76 (2004) 1134. 
Very long, high-resolution SCX capillary with very reproducible proteomics results with plasma.  
S126)
Resing et al., Anal. Chem. 76 (2004) 3556. 
Proteomics of K562 erythroleukemia cells: Improved peptide validation algorithm.  
S127)
Liu et al., Anal. Chem. 76 (2004) 5345. 
Enrichment in cysteine-containing peptides via covalent chromatography as an alternative to ICAT for proteomics.  
S128)
Rejtar et al., Anal. Chem. 76 (2004) 6017. 
Improved data processing to improve peptide identification in proteomics.  
S129)
Maynard et al., J. Chromatogr. B, 810 (2004) 69. 
Development of a multidimensional LC-MS system for proteomics, with a yeast model sample.  
S130)
Kang and Frey, Biotechnol. Bioeng. 87 (2004) 378. 
Chromatofocusing of proteins and peptides on PolySULFOETHYL A columns.  
S131)
Gilar et al., J. Chromatogr. A, 1061 (2004) 183. 
Peak capacity in 2-D LC investigated (with suboptimal gradients).  
S132)
Beausoleil et al., Proc. Natl. Acad. Sci. 101 (2004) 12130. 
Selective isoln. of phosphopeptides from tryptic digests of HeLa cell nuclear 
proteins using PolySULFOETHYL A with 200- pores.
S133)
Ballif et al., Mol. Cell. Prot. 3 (2004) 1093. 
Selective isoln. of phosphopeptides from embryonic mouse brain using PolySULFOETHYL A with 200- pores.  
S134)
Hardwidge et al., J. Biol. Chem. 279 (2004) 20127. 
Proteomics of intestinal epithelial cell response to E. coli.  
S135)
Kao et al., J. Biol. Chem. 279 (2004) 51554. 
Proteomics of metabolic regulation by copper ions in Methylococcus capsulatus.  
S136)
Jacobs et al., J. Proteome Res. 3 (2004) 68. 
Proteomics of human mammary epithelial cells.  
S137)
Fujii et al., J. Proteome Res. 3 (2004) 712. 
2-D LC analysis of human plasma proteome.  
S138)
Strittmatter et al., J. Proteome Res. 3 (2004) 760. 
Correlation of peptide retention time in LC with identification in proteomics.  
S139)
Blonder et al., J. Proteome Res. 3 (2004) 862. 
Blonder et al., J. Proteome Res. 3 (2004) 862.  
S140)
Lu et al., J. Proteome Res. 3 (2004) 949. 
Riboproteomics of Hepatitis C virus ribosomal entry site.  
S141)
Wilmarth et al., J. Proteome Res. 3 (2004) 1017. 
2-D LC proteomics of human saliva.  
S142)
Sethuraman et al., J. Proteome Res. 3 (2004) 1228. 
Redox proteomics: ICAT identification of oxidant-sensitive cysteines in complex protein mixtures.  
S143)
Xiang et al., J. Proteome Res. 3 (2004) 1278. 
Proteomics of membrane proteins from breast cancer cell lines MCF7 and BT474.  
S144)
Himeda et al., Mol. Cell. Biol. 24 (2004) 2132. 
Proteomic identification of Six4 as the Trex-binding factor in the muscle creatine kinase enhancer.  
S145)
Glowacki and Jakubowski, J. Biol. Chem. 279 (2004) 10864. 
Derivatization of lysine residues in HSA by homocysteine is affected by cross-talk with a cysteine residue.  
S146)
Chwatko and Jakubowski, Clin. Chem. 51 (2005) 408. 
Urinary excretion of homocysteine-thiolactone in humans.  
S147)
Martin et al., Cancer Res. 64 (2004) 347. 
Proteomics of neoplastic prostate epithelium.  
S148)
Streit et al., J. Invest. Dermatol. 105 (1995) 562. 
Isoln. from urine of protease inhibitors elafin and bikunin.  
S149)
von Haller et al., Mol. Cell. Prot. 2 (2003) 428. 
Statistical tools for data analysis in ICAT.  
S150)
Reeve et al., Am. J. Physiol. Gastrointest. Liver Physiol. 287 (2004) G326. 
Purif. of nonsulfated cholecystokinin-58 from canine intestine.  
S151)
Masuda et al., J. Chromatography A, 1063 (2005) 57. 
Optimization of an automated microscale system for 2-D LC in proteomics.  
S152)
Malmstršm et al., J. Chromatogr. B, 815 (2005) 333. 
ICAT analysis of fibroblast nuclear proteins after stimulation with heparan sulfate.  
S153)
Lee et al., Proteomics 3 (2003) 2472. 
Proteomics of hepatoma cells; even a little pre-digest fractionation of intact proteins increases peptide 
identification dramatically in shotgun proteomics.
S154)
Wang et al., Analyst 127 (2002) 1450. 
RPC-SCX-RPC proteomics analysis of P. gingivalis bacteria.  

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PAPERS on PolyHYDROXYETHYL® Aspartamide


February 22, 2005    HILIC and SEC
Ref#
SOURCE 
Background reference: 
H0)
Bosch et al., Anal. Chem. 68 (1996) 3651-3657.
Retention of Ionizable Compounds on HPLC. pH Scale in Methanol-Water and the pK and pH Values of Buffers.
H00)
Canals et al., Anal. Chem. 72 (2000) 1802.
Retention of Ionizable Compounds on HPLC. 4. Mobile-Phase pH Measurement in Methanol/Water.
Ref#
SOURCE
SUBJECT
H1)
 Alpert, J. Chromatogr. 499 (1990) 177.
Introduction of HILIC for HPLC of polar compounds.
H2)
 Zhu et al., J. Chromatogr. 548 (1991) 13.
Peptide HILIC on PolyHYDROXYETHYL A and PolySULFOETHYL A (same ref. as  S16).
H3)
 Fainsilber et al., Eur. J. Biochem. 202 (1991) 589.
Size-Exclusion HPLC of a conotoxin peptide.
H4)
 Andrews & Alpert, Poster #110, 10th ISPPP (10/90)
Size-Exclusion HPLC  of small solutes.
H5)
 Pike et al., Proc. Natl. Acad. Sci. 88 (1991) 11081.
Lactic acid stimulates B-cell growth.
H6)
 Przysiecki et al., Protein Expression Purif. 3 (1992) 185.
HILIC, SEC, and SCX of recombinant antistasin with a preproleader sequence same ref. as S18).
H7)
 Swiderek et al., Poster #M93, Protein Society  Symp. (7/92).
Isoln. of carcinoembryonic antigen glycopeptides by HILIC, with mass spec. sequencing.
H8)
 Boutin et al., J. Chromatogr. 583 (1992) 137.
HILIC of phosphorylated peptides and tyrosine kinase reaction mixtures.
H9)
 Fong et al., Plant Physiol. 99 (1992) 548.
SEC of gymnosperm Ser-Hyp4 motif protein (same ref. as S26).
H10)
Hogrefe et al., Nucl. Acids Res. 21 (1993) 2031.
HILIC of methylphosphonate oligonucleotides.
H11)
Bickel et al., Proc. Natl. Acad. Sci. 90 (1993) 2618.
Purif. of biotinylated VIP analog.
H12)
Horst et al., EMBO Journal 12 (1993) 3035.
HILIC of mitochondria proteins from SDS-PAGE gel bands.
H13)
Clauser and Burlingame, Poster M286, Protein Society Symp. (7/93)
Removal of SDS from proteins via capillary HILIC.
H14)
Boutin et al., Drug Metab. Disp. 21 (1993) 1157.
HILIC of glucuronides of diosmetin.
H15)
Barret et al., Chem.-Biol. Interact. 86 (1993) 17.
Assay of ATPase activity by HILIC.
H16)
Jeno et al. Anal. Biochem. 215 (1993) 292.
Removal of SDS and salts from electroeluted proteins.
H17)
Soltysik et al., Ann. NY Acad. Sci. 690 (1993) 392.
Purif. of saponins by HILIC.
H18)
Alpert et al., J. Chromatogr. A 676 (1994) 191. 
PolyGLYCOPLEX for HILIC of complex carbohydrates.
H19)
Swiderek et al., Poster 268-M, Protein Society Symp. (7/94).
HILIC for removal of SDS, Triton X-100, and Nonidet P40 from peptides on-line for LC-MS.
H20)
Alpert et al., Poster 269-M, Protein Society Symp. (7/94).
HILIC of intact proteins.
H21)
Harwig et al., Meth. Enzymol. 236 (1994) 160.
Purif. by HILIC of defensins.
H22)
Ernould et al., Int. J. Peptide Protein Res. 43 (1994) 496.
HILIC of phosphorylated peptides from tyrosine kinase reactions.
H23)
Taylor et al., J. Am. Chem. Soc. 116 (1994) 10803.
Isoln. of dihydroxyproline from mussel adhesive protein.
H24)
Silvestre et al., J. Agric. Food Chem. 42 (1994) 2778.
Fractionation of casein hydrolyzates by SEC.
H25)
Silvestre et al., J. Agric. Food Chem. 42 (1994) 2783.
Characterization by SEC of casein hydrolyzates.
H26)
Shushan, Perkin-Elmer "Views", Fall 1994, 16-17.
SEC desalting of proteins for ES-mass spec.
H27)
Furuya et al. Proc. Natl. Acad. Sci. 92 (1995) 12323.
Isoln. by RPC/HILIC of a diuretic hormone polypeptide from mealworm (T. molitor).
H28)
Kieliszewski et al., J. Biol. Chem. 270 (1996) 2541.
HILIC of hydroxyproline-rich glycopeptides from Douglas Fir, with MS to determine sites of arabinoside attachment.
H29)
Sarg et al., Chromatographie 1/95 (1995) 50.
HILIC of histones (using PolyCAT A).
H30)
Oyler et al., J. Chromatogr. A 724 (1996) 378.
HILIC of diastereomers of atosiban.
H31)
Pauly et al., Carbohydr. Res. 282 (1996) 1.
HILIC of nitrobenzyl- derivs. of oligo-saccharides on PolyGLYCOPLEX.
H32)
Horst et al., Meth. Enzymol. 260 (1995) 232.
Isoln. of yeast mitochondrial membrane proteins from 2-D gels by HILIC.
H33)
Lindner et al., J. Chromatogr. A 743 (1996) 137.
 HILIC of acetylated histones (using PolyCAT A).
H34)
Nelson et al., Exp. Cell Res. 229 (1996) 20.
Isoln. of a hepatic growth stimulator by SEC; it's glycerophosphorylethanolamine.
H35)
Lindner et al., J. Chromatogr. A. 782 (1997) 55.
 HILIC of phosphorylated histones using PolyCAT A (same ref. as C64).
H36)
Swiderek et al., ABRF News 8(4) (1997) 17.
Analysis of protein and peptide samples that contain detergents.
H37)
Lane et al., J. Cell Biol. 125 (1994) 929.
Analysis of the peptides GHK and KGHK in digests of SPARC protein.
H38)
Swiderek et al., Meth. Enzymol. 271 (1996) 68.
Separation of glycopeptides from digest of carcinoembryonic antigen by RPC-HILIC.
H39)
Mant et al., Meth. Enzymol. 289 (1997) 426.
CEX-HILIC of peptides (using PolySULF. A).
H40)
Schmerr et al., J. Chromatogr. A 802 (1998) 125.
Purif. and analysis of the scrapie prion protein (using PolyWAX LP) [same ref. as W5].
H41)
Lindner et al., J. Biol. Chem. 273 (1998) 13324.
HILIC of deamidation and acetylation variants of H1 histone (using PolyCAT A).
H42)
Strege, Anal. Chem. 70 (1998) 2439.
HILIC-ES-MS of small polar compounds in drug discovery screens.
H43)
Zhang and Wang, J. Chromatogr. B 712 (1998) 73.
HILIC of coeluting glycopeptides from an RPC map of a tryptic digest of recombinant g-interferon from a CHO cell culture.
H44)
Faull et al., Neuropeptides 32 (1998) 339.
RPC-HILIC-CEX isolation of g-lipotropin and b-endorphin from rat pituitary.
H45)
Mant et al., J. Chromatogr. A 816 (1998) 65.
CEX-HILIC detects substitutions on the polar face of amphipathic a-helical peptides; 
RPC, the nonpolar face (PolySULF. A used).
H46)
Mant et al., J. Chromatogr. A 816 (1998) 79.
CEX-HILIC vs. RPC for separation of variants of cyclic peptides (Gramicidin S analogs)
(PolySULF. A used).
H47)
Alpert, "Size Exclusion HPLC of Small Solutes,"Column Handbook for 
Size Exclusion Chromatography
(C.-S. Wu, ed.), Academic Press,1999.
Explains the principles and gives applications. 
H48)
Strege, Am. Pharmaceut. Rev. 2 (1999) 53.
HILIC-MS combination for drug discovery.
H49)
Litowski et al., J. Pept. Res. 54 (1999) 1 
HILIC-CEX can resolve peptides that vary in position of Ser- acetylation (PolySULF. A used).
H50)
Croes et al., Poster 134, 47th ASMS Conference (June 1999; Dallas)
HILIC of underivatized amino acids in seeds with MS/MS detection; run time of 6 minutes.
H51)
 Dahiyat and Mayo, Proc. Natl. Acad. Sci. 94 10172.
SEC of designed proteins @ packing interactions.
H52)
 Serrano et. al., J. Biol. Chem. 270 (1995) 27244.
HILIC with PolyGLYCOPLEX of the carbohydrates from a GPI anchor of Trypanosoma cruzi.
H53)
 Ke et al., J. Biol. Chem. 272 (1997) 16603.
HILIC of small, polar peptide products from t-PA and urokinase substrate cleavage.
H54)
 Ke et al., J. Biol. Chem. 272 (1997) 20456.
HILIC of small, polar peptide products from a high-affinity urokinase substrate library.
H55)
 Kisselev et al., J. Biol. Chem. 273 (1998) 1982.
SEC of peptides generated by degradation of model proteins by archaeal proteasomes.
H56)
 Kisselev et al., J. Biol. Chem. 274 (1999) 3363.
SEC of peptides generated by degradation of model proteins by mammalian proteasomes.
H57)
 Dallet et al., J. Chromatogr. B, 742 (2000) 447.
HILIC of urea, allantoin, and lysine pyroglutamate in cosmetics.
H58)
 Strege et al., Anal. Chem. 72 (2000) 4629.
HILIC of small molecules with coupled anion- and cation-exchange columns.
H59)
 Mizzen at al., J. Chromatogr. B, 744 (2000) 33.
Sepn. of Histone H1 variants by HILIC on PolyCAT A.
H60)
 Hindi-Tamelikecht et al., J. Agric. Food Chem. 45 (1997) 4758.
SEC analysis of peptide size in chickpea protein hydrolyzates.
H61)
 Dahiyat et al., Protein Sci. 6 (1997) 1333.
SEC of engineered helical proteins: distinguishing dimers from tetramers.
H62)
 Rabearimonjy et al., in Proc. 9th Intl. Symp. Cyclodextrins, 
pg. 19 (1999); Kluwer Academic Publishers; Dordrecht, The Netherlands.
HILIC of hydroxypropyl cyclodextrins.
H63)
 Sarg et al., Arch. Biochem. Biophys. 372 (1999) 333.
HILIC of avian Histone H5; degree of N-term. acetylation vs. age.
H64)
 Wang et al., Poster, ASMS 1999.
HILIC of NAD in cell extracts following use of HILIC SPE cartridge to get rid of perchloric acid.
H65)
 Doucette et al., Toxicon 38 (2000) 1465.
Purif. of synthetic 11-[3H]-tetrodotoxin.
H66)
 McGrane et al., EuroResidue IV (May 2000), Pg. 765 (van Ginkel
& Ruiter, eds.), Bilthoven, The Netherlands.
HILIC-MS for analysis of aminoglycoside antibiotic residues.
H67)
 Scrofani et al., Protein Sci. 9 (2000) 2018.
Purif. of vascular endothelial growth factor-B. 
H68)
 Kšhler et al., Molec. Cell 7 (2001) 1143.
SEC of small peptides in proteasome studies: their fluorescamine derivs. elute more ideally.
H69)
 Troyer et al., J. Chromatogr. A, 919 (2001) 299.
HILIC of intact glucosinolates from broccoli.
H70)
 Chen et al., Eur. J. Biochem. 251 (1998) 691.
HILIC and RPC of neutral N-glycans in rat brain tissue.
H71)
 Zal et al., Glycobiology 8 (1998) 663.
HILIC of N-linked glucose-capped oligosaccharides from hemoglobins of a deep-sea tube worm.
H72)
 Ye et al., LCGC 19 (2001) 1076.
HILIC-CEC of polar compounds (used PolySULF. A).
H73)
Garbis et al., Anal. Chem. 73 (2001) 5358-62. 
HILIC of folic acid metabolites in plasma with direct LC-MS/MS detection.
H74)
 Cascio et al., EMBO J. 21 (2002) 2636.
26S Proteasome studies: SEC of small peptides.
H75)
 Byrne et al., Bioconj. Chem. 13 (2002) 571.
Analysis by HILIC on PolyGLYCOPLEX of an galactosyl oligosaccharide used as an immune response blocker in xenotransplantation.
H76)
 Stršmqvist, J. Chromatogr. A, 667 (1994) 304.
SEC-RPC of a tryptic digest of superoxide dismutase.
H77)
 Curren & King, J. Chromatogr. A, 954 (2002) 41.  
HILIC analysis and SPE extraction of avoparcin from kidney extracts. 
H78)
 Vanderlaan et al., J. Chromatogr. A, 711 (1995) 23.
SEC to isolate neural acetylcholinesterase (280 KDa) for online assay.
H79)
 Kett et al., Carboh. Res. 329 (2000) 169.
Purification of sugar pyrazolone derivs.
H80)
 Litowski & Hodges, J. Biol. Chem. 277 (2002) 37272.
SCX/HILIC purification of peptides to eliminate acetylated serine impurities.
H81)
 Fu et al., Electrophoresis 24 (2003) 2084.
HILIC-CEC of peptides with  PolySULFOETHYL A.
H82)
 MacCoss et al., Proc. Natl. Acad. Sci. USA 99 (2002) 7900.
HILIC-SCX-RPC capillaries for 3-D proteomics analysis of lens tissue proteins.
H83)
 Moyer et al., Clin. Chem. 48 (2002) 1460.
HILIC-MS/MS of nicotine and its metabolites in serum or urine.
H84)
 Hartmann et al., J. Chromatogr. A, 1009 (2003) 61.
RPC vs. HILIC-SCX for separation of subst. helical peptides.
H85)
 Fahey et al., J. Chromatogr. A, 996 (2003) 85.
Prep-scale purif. of glucosinolates from seeds.
H86)
 Wu et al., Nat. Biotechnol. 21 (2003) 532.
HILIC-SCX-RPC capillaries for 3-D proteomics analysis of membrane proteins.
H87)
Agrwal et al., J. Biol. Chem. 268 (1993) 14932. 
SEC of recombinant carbohydrate-binding protein 35. 
H88)
Phister et al., Appl. Env. Microbiol. 70 (2004) 631. 
Analysis via RPC-SEC-ESI-MS of bacilysin, chlorotetraine, and iturin A produced by bacteria in fermented maize dough. 
H89)
Karlsson et al., J. Chromatogr. Sci. 42 (2004) 361. 
HILIC of sucrose with ELSD detection. 
H90)
Wu et al., Anal. Chem. 76 (2004) 4951. 
HILIC-SCX-RPC capillaries for 3-D proteomics analysis of rat proteins. 
H91)
Cheung et al., J. Nucl. Med. 45 (2004) 867. 
SEC of a single chain Fv antibody + strepavidin fusion protein. 
H92)
Fu et al., Electrophoresis 25 (2004) 600. 
HILIC-CEC with PolySULFOETHYL A for anal. of basic drugs in human serum. 
H93)
Kulanthaivel et al., J. Biol. Chem. 279 (2004) 36250. 
Purif. of lipoglycopeptide antibiotics by HILIC. 
H94)
Saric et al., J. Biol. Chem. 279 (2004) 46723. 
SEC to follow degradation of post-proteasomal peptides. 
H95)
Held et al., J. Biol. Chem. 279 (2004) 55474. 
SEC sepn. of tyrosine, isodityrosine, and di-isodityrosine in extensin analog crosslink study. 
H96)
Hodges et al., J. Chromatogr. A, 1053 (2004) 161. 
HILIC vs. RPC for monitoring the polarity of amino acid side chains in polar and nonpolar faces of amphipathic a-helices. 
H97)
Wu et al., Mol. Biol. Cell 15 (2004) 2907. 
Triphasic MuDPIT capillary (HILIC on top) for proteomics of Golgi proteins. 
H98)
Schmerr and Alpert, in Prions and Mad Cow Disease (Nunnally & Krull, eds.), 
Marcel Dekker, 2004, pp. 359-377.
Detection of the prion protein in blood using HILIC extraction in a fluorescence immunoassay. 
H99)
Zywicki et al., Anal. Biochem. 336 (2005) 178. 
HILIC vs. RPC in metabolomics. 
H100)
Darlington and Gann, J. Trauma 58 (2005) 1. 
Adenosine isolated from plasma via HILIC and shown to reverse hemorrhagic shock by stimulation of Na/K ATPase. 
H101)
Kieliszewski et al., Plant J. 5 (1994) 849. 
SEC isoln. of HyPro-rich fragments from potato lectin. 
H102)
McCarthy et al., J. Neurochem. 70 (1998) 1950. 
Isoln. of Met-oxidized Neuropeptide Y from hippocampus. 

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PAPERS on Poly(ALKYL)® Aspartamide


February 22, 2005	PolyPROPYL-, -ETHYL, and -METHYL Aspartamide®   Hydrophobic Interaction Chromatography (HIC)
Ref#
SOURCE
SUBJECT
P1)
Lachman et al., Meth. Enzymol. 116 (1985) 467.
Purif. of Interleukin-1 with PolyPROPYL A.
P2)
Alpert, J. Chromatogr. 359 (1986) 85.
Prepn. and use of PolyPROPYL-, -ETHYL, and -METHYL A. 
P3)
Wetlaufer and Koenigbauer, J. Chromatogr. 359 (1986) 55.
Hydrophobic-Interact. Chromat. (HIC) of proteins using surfactants.
P4)
Yu and Glazer, J. Biol. Chem. 262 (1987) 17543.
Purif. of tyrosine kinase from HL-60 cells.
P5)
Alpert, BioChromatography 2 (1987) 131.
Peptide HPLC: HIC vs. RPC.
P6)
Alpert, J. Chromatogr. 444 (1988) 269.
Peptide HPLC; HIC vs. RPC.
P7)
Benedek, J. Chromatogr. 458 (1988) 93.
HIC of lactalbumin: Denaturation and retention vs. temp. and stationary phase.
P8)
Rosenstreich et al., J. Exp. Med. 168 (1988) 1767.
Purif. of an interleukin-1 inhibitor.
P9)
Wu et al., J. Chromatogr. 500 (1990) 595.
Assay of recombinant human growth hormone with various columns.
P10)
Meyer et al., Biochem. J. 274 (1991) 409.
Purif. of a new class (Theta) of glutathione transferases.
P11)
Retzios & Markland, Biochemistry 31 (1992) 4547.
Purif. of fibrinolytic enzymes from rattlesnake venom.
P12)
Trikha et al., Thrombosis Res. 73 (1994) 39.
Purif. of platelet aggregation inhibitors from snake venoms.
P13)
Loayza et al., J. Chromatogr. B 662 (1994) 227.
Resoln. of isoforms of fibrinolytic enzyme from copperhead snake venom.
P14)
Welinder et al., Anal. Chem. 67 (1995) 39A.
Systematic study of column variability in HPLC of human growth hormone derivative with PolyPROPYL A and other columns.
P15)
Retzios et al., Protein Exp. Purif. 1 (1990) 33.
Purif. of fibrinolytic enzymes from copperhead snake venom.
P16)
Ellis et al., Blood Coag. Fibrinol. 4 (1993) 537.
Isoln. of fibrinolytic fragments D-dimer, D1 & E with PolyETHYL A.
P17)
Mikolajczyk et al., Bioconjugate Chem. 5 (1994) 636.
Purif. of derivs. of b-lactamase and a murine Fabí antibody fragment.
P18)
Kumar et al., Cancer Res. 57 (1997) 3111.
Sepn. of prostate-specific antigen from its pro- form after conversion by kallekrein.
P19)
Mikolajczyk et al., Urology 50 (1997) 710.
Sepn. of prostate-specific antigen from several pro- forms and PSA-ACT complex.
P20)
Mikolajczyk et al., Eur. J. Biochem. 246 (1997) 440.
Sepn. of prostate-specific human kallekrein 2 from its pro- form.
P21)
Woodrum et al., Clin. Chem. 43 (1997) 1203.
Purif. of prostate-specific antigen from seminal plasma for use in an immunoassay.
P22)
Mikolajczyk et al., Urology 55 (2000) 41.
Isoln. of a clipped PSA variant (BPSA) correlated with benign prostate disease.
P23)
Mikolajczyk et al., Cancer Res. 60 (2000) 756.
Correlation of proPSA with prostate cancer.
P24)
Wang et al., Eur. J. Biochem. 267 (2000) 4040.
Antibodies to BPSA and PSA don’t cross react well.
P25)
Mikolajczyk et al., The Prostate 45 (2000) 271.
Identification of BPSA in seminal plasma.
P26)
Somers et al., Structure 6 (1998) 1601.
Purif. of GDP-fucose synthetase from E. coli.
P27)
Menon et al., J. Biol. Chem. 274 (1999) 26743.
Purif. of GDP-fucose synthetase from E. coli.
P28)
Hunter et al., Mol. Biochem. Parasitol. 103 (1999).
Purif. of recomb. SAG1 surface protein from Toxoplasma gondii.
P29)
Meyer et al., Biochem. J. 313 (1996) 223.
Purif. of prostaglandin-H E-isomerase from the nematode Ascaridia.
P30)
Keith et al., Biotechnol. Appl. Biochem. 34 (2001) 5.
Protein measurement in tears to determine their adhesion to contact lenses and vials.
P31)
Tanaka et al., Invest. Opthalmol. Vis. Sci. 45 (2004) 245.
Isoln. of a growth-promoting factor for retinal pigment epithelial cells via IEX-HIC-RPC.
P32)
Linton et al., Clin. Chem. 49 (2003) 253.
Purif. of benign prostate-specific antigen (BPSA).
P33)
Wiesenborn et al., Appl. Env. Microbiol. 55 (1989) 317. 
AEX-HIC purif. of phosphotransbutyrylase from C. acetobutylicum. 
P34)
Wiesenborn et al., Appl. Env. Microbiol. 55 (1989) 323. 
Purif. by HIC of CoA transferase from C. acetobutylicum. 
P35)
Mikolajczyk et al., Clin. Chem. 50 (2004) 1017. 
HIC of proenzyme forms of PSA in improved detection of prostate cancer. 
P36)
Lai et al., Life Sci. 76 (2005) 1267. 
Purif. by HIC of holotransferrin tagged with artemisinin for targeting cancer cells. 

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PAPERS on PolyWAX LP®

February 22, 2005	Weak Anion Exchange (WAX)
Ref#
SOURCE
SUBJECT
W1)
Alpert and Regnier, J. Chromatogr. 185 (1979) 375.
Preparation of the precursor of PolyWAX LP for anion-ex. HPLC of proteins.
W2)
Baxter et al., J. Biol. Chem. 264 (1989) 11843. 
Purif. of acid-labile subunit (ex human serum) from insulin-like growth factor binding complex.
W3)
Ronk et al., Tech. Protein Chem. 5 (1994) 259.
 Anion-exchange of proteins with packed capillaries.
W4)
Baxter and Dai, Endocrinology 134 (1994) 848.
 Purif. of acid-labile subunit (ex rat serum) from insulin-like growth factor binding complex.
W5)
Schmerr et al., J. Chromatogr. A 802 (1998)135.
 Purif. and analysis of the scrapie prion protein (same ref. as H40).
W6)
Azhayev & Antopolsky, Tetrahedron 57 (2001) 4977.
 Analysis of phosphorothioate oligonucleotide analogs.
W7)
Hernandez et al., J. Virol. Meth. 120 (2004) 141. 
Rapid purif. of GC-rich PCR products by anion-exchange instead of gels. 
W8)
Zhang et al., J. Biol. Chem. 279 (2004) 50969. 
Use of a PolyWAX cartridge for desalting acyl carrier protein with a volatile solvent. 
W9)
Leonardi et al., J. Biol. Chem. 280 (2005) 3314. 
Use of a PolyWAX cartridge for desalting pantothenate kinase with a volatile solvent. 
W10)
Kotlyar et al., Nucl. Acids Res. 33 (2005) 525. 
PolyWAX LP for purification of poly(dC) oligonucleotides and to analyze dNTP's. 

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PAPERS on PolyGlycoplex®

February 22, 2005	HPLC of Complex Carbohydrates
Ref#
SOURCE
SUBJECT
H18)
Alpert et al., J. Chromatogr. A 676 (1994) 191. 
PolyGLYCOPLEX for HILIC of complex carbohydrates.
H31)
Pauly et al., Carbohydr. Res. 282 (1996) 1.
HILIC of nitrobenzyl- derivs. of oligo-saccharides on PolyGLYCOPLEX.
H52)
Serrano et. al., J. Biol. Chem. 270 (1995) 27244.
HILIC with PolyGLYCOPLEX of the carbohydrates from a GPI anchor of Trypanosoma cruzi.
H75)
Byrne et al., Bioconj. Chem. 13 (2002) 571.
Analysis by HILIC on PolyGLYCOPLEX of an galactosyl oligosaccharide used as an immune response blocker in xenotransplantation.

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