SDS Removal with HILIC microSPE Columns
SDS Removal with HILIC microSPE Column
For your convenience, we accept Mastercard,
VISA, and American Express credit cards.
| Home
| IdeaBook
| Orders
| Price/Applic
| Price/Vendor
| Protocols|
Peptide sequencing labs frequently use sodium dodecyl sulfate (SDS) to solubilize
peptides and proteins from electrophoresis gels. Unfortunately, the SDS in the
samples often ruins subsequent runs by reversed-phase HPLC (RPC). Moreover, in
automated peptide sequencers, SDS in samples can lead to bubble formation in the
sample intake lines, which blocks further sample intake. SDS can also accumulate
on the PVDF membranes used for sequencing, washing off at an inconvenient time.
All in all, it's a good idea to get it out of your samples.
Use of a HILIC HPLC Jeno's technique for SDS or
other detergents, or by pre-treatment
of the sample with a HILIC MiniSpin micro-SPE Tip (p/n: SUM HIL) can remove
detergents and contaminating dyes prior to injection onto your RPC column.
In this example, 8.6% SDS was removed by HILIC from a peptide mixture prior to an RPC analysis with a HILIC microSPE tip from Glygen, Corp..
Data courtesy of Britt-Marie Olsson (Stockholm Univ.)
The size of the microSPE column is determined by the capacity needed for the retained
molecules only, since the SDS comes off during the loading (in 60-80% ACN) and does not
take up capacity on the HILIC cartridge. Peptide is eluted at 100% water 30mM ammonium formate
and is thus ready for direct RPC loading, or for direct ESI-MS analysis.
See Spin columns for part numbers
PUMSCHY1203 or PMASCHY1203 for individual Spin Columns or
UltraMicroSpin or MicroSpin for packages of
HILIC MiniSpin columns/Tip columns, SUM HIL, STP HIL and SEM HIL.
Copyright© 1995-2024 The Nest Group, Inc.™ All rights reserved (established 1984)
For more information or to place
an order contact:
The Nest Group, Inc.™ 17 Hayward St., Ipswich, MA 01938-2041 USA
Tel: 1-508-481-6223
Fax: 1-508-485-5736
For your convenience, we accept Mastercard,
VISA, and American Express credit cards.
| Home
| IdeaBook
| Ordering
| Price/Applic
| Price/Vendor
| Protocols|
If you have problems or comments concerning
our WWW service, please send an e-mail to
webmaster.
About Us | GDPR Privacy Policy | Trademarks | Contact Us
Last Updated: 08/20/21