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Below is a chronological list of events:
 

  • 3/12/08 - Added the reference Science, 319, 1197-98 (2008) to the Protein HIC section for a discussion of Specific Ion Effects regarding the hydration of ions on surfaces.
  • 2/20/08 - Added PROTO300 C4: Comparison to Vydac 214TP C4 for proteins.
  • 2/19/08 - Added Ion Chromatography: Anion Membrane Suppressor (SAMS™) and the Continuous Anion Regeneration System (CARS™) columns.
  • 12/31/07 - Added YMC America as a New source for a broad range of HPLC and Process scale bonded medias.
  • 12/20/07 - Added Comparison of the All-Guard™ Cartridges to the Vydac "...GD54" Guard Cartridges.
  • 11/7/07 - Added Brian Hampton's ASMS Poster Regarding The Importance of Contact Time for Either Desalting or Trapping of Polar Solutes on Trap Columns.
  • 11/1/07 - Added KOT Knitted Open Tubular Reactors to price list.
  • 7/14/07 - Added ERLIC Discussion of Electrostatic Repulsion Effects in HILIC separations.
  • 2/14/07 - Added Newly Designed Index, Protocols and Personnel pages. We hope you like it better
  • 12/5/06 - Added PROTO™ 200 & PROTO™ 300 & Peptide & Protein PROTEO columns to Higgins Analytical section of Price List
  • 11/1/06 - Added Buffer Solubility in Aqueous-Organic Solvents Useful for RPC and/or HILIC mobile phase composition selection.
  • 8/25/06 - Added Dialysis Bibliography to Dialysis section of Protocols
  • 8/10/06 - Added [sic] "HPLC Method for the Determination of Pharmaceutical Salts" to ZIC-HILIC section of Protocols
  • 5/15/06 - Added Basics of HPLC to Training section of Protocols
  • 5/12/06 - Added WWW Chemistry Guide - a collection of links for organic, biochemical, computational, medicinal and other chemistry research or education.
  • 5/2/06 - Added 110nL capillary LC/MS Guard/Traps to Higgins Analytical Piccolo™ price list
  • 7/20/05 - Added a search engine to the site for searching within The Nest Group URL, www.nestgrp.com .
  • 7/6/05 - Added ZIC-pHILIC® for Adenosine Phosphate Analysis (AMP, cAMP, dAMP, ADP, dATP and ATP) Using HILIC Conditions with Volatile Buffers (70% ACN, 30% Ammonium Carbonate, pH 8.8, 100 mM).
  • 5/6/05 - Added comparison (Y. Guo & S. Gaiki, J. Chromatogr. A, 1074 (2005) 71-80.) of Amide, Amine, Silica and betain sulfonate functional groups for HILIC separations of small polar acids, nucleic acid bases and nucleosides.
  • 4/9/05 - Added Oleg Krokhin's Peptide RT Calculator. New sequence-specific correction factors for prediction of peptide retention in RP-HPLC: application to protein identification by off-line HPLC-MALDI-MS.
  • 3/19/05 - Added references for measurement of pH in aqueous/organic solutions
  • 3/7/05 - Updated PolyLC Bibliographic references
  • 2/04/05 - Added this ExPASy link in Chemistry WWW Resources to Calculate pKa and provide other information resources for life scientists with an interest in biological macromolecules. Links to protein sequence, 3D structure and 2D-gel analytical tools are provided on the ExPASy server, and more specifically from its Proteomics tools page.
  • 11/01/04 - Added this link in Chemistry WWW Resources. The Hofmeister Effect and the implications for HIC. This site discusses the ranking of various ions toward their ability to precipitate a mixture of proteins.
  • 06/03/04 - Added pdf HILIC Comparison to RPC for Metabolomics.
  • 03/05/04 - Added slides: Designing a purification protocol using ion exchange resins.
  • 10/17/03 - Added chromatograms on Aminoglycoside (Gentamycin) Antibiotic separations in the Protocols Index under Polar Molecule and Detergent Removal by HILIC.
  • 4/18/03 - Added a series of articles on Mobile-Phase Buffer considerations in the Protocols Index under Training.
  • 4/1/03 - New Everest™ C18 Ultra High Resolution Tryptic Peptide Mapping Columns. LC-MS/MS Capillaries, Guards and Analytical Columns.
  • 3/15/03 - Added New Higgins Capellini™, Piccolo™ and Sprite™ LC-MS/MS Capillaries, Guards and Trap Columns.
  • 9/19/2002 - HILIC LC-MS/MS of Folic Acid Metabolites and Underivatized Amino Acids.
  • 7/8/2002 - PAGE*Eraser™ microFilter Spin Columns remove particulates from samples from in-gel digests to prolong capillary LC-MS column life.
  • 6/20/2002 - Added Catalog of HAISIL® HPLC Columns and Cartridge Products. Complete (2,668K pdf) or individual sections: HAISIL 100® (small pore) and HAISIL 300® (wide pore) products (Type A Silicas); and TS TARGA ®, CS CLIPEUS® and LS "High Load" products (Type B Silicas).
  • 4/8/2002 - Added Hydrophobic Interaction (HIC) part numbers and prices.
  • 4/5/2002 - Dialysis membrane conditoning and solvent compatibility information.
  • 3/6/2002 - Proteomics applications newsletter on low TFA MS Vydac media using prepacked
  • capillary columns from 0.075mmID - 0.500mmID.
  • 2/14/2002 - Training lecture: Basics of Dialysis and Ion Exchange, added to Nest Protocols Section
  • 2/13/2002 - Orbigen Biology Protocols link added to WWW Biology Sites section
  • 1/22/2002 - Biology Online link added to our WWW Biology Sites information section.
  • 10/31/2001 - New! Grace Vydac 0.075 and 0.150mmID Capillary Columns for "Low TFA" LC-MS operation enhace detection of peptides.
  • 9/10/2001 - Proteomics: Pi3™ TYR-P Poster data. Isolation of tyrosine-phosphate containing peptides from digests
  • 5/1/2001 - Proteomics Fractionation: Pi3™ amino acid specific resins for complex sample fractionation
  • 3/14/2001 - HIC and IEX column care added to IdeaBook and Protocols sections for PolyLC products.
  • 3/1/2001 - 2-D chromatographic fractionation of 1,500-2000 peptides per run at 5 orders of magnitude better detectability than 2-D gel electrophoresis whether you are using Multidimensional Chromatographic Analysis of Proteins (MUDCAP), Signature Peptides, the DALCPC method (Direct Analysis of Large Protein Complexes), ICAT™ (isotope-coded affinity tagged) labeled, or isolating specific amino acid containing peptides from digested proteins for proteomic characterization.
  • 1/10/2001 - The Nest Group New URL established to utilize cgi scripting and dynamic HTML.
  • 8/22/2000 - Pdf technical sheets were integrated with the ideabook section for all products.
  • 1/12/2000 - Updated all products with new prices.
  • 11/30/99 - SuproTip microSPE Tip columns were added.
  • 3/18/99 - MiniSpin Columns and MicroTip Kits were added.
  • 9/1/98 - Biology Reference sites were added.
  • 6/10/98 - DispoBioDialyser AmiKa's DispoBioDialyser ® disposable, small volume (10-100 ul) dialysis kits added.
  • 6/10/98 - Cozap AmiKa's Cozap® Coomassie Blue destaining products were added.
  • 11/15/96 - New, EXCLU-SIEVE® Agaroses are added to the protocols section.
  • 6/25/96 - Vydac Cross Reference Guide is added.
  • 2/1/96 - The Nest Group, Inc. now accepts credit card orders for your convenience.
  • 1/31/96 - The Nest Group, Inc. URL received the Magellan 4 Star Award.
  • 12/13/95 - Vydac RPC Buffer Note "Validation of Reverse Phase Chromatography separations (TFA, PO4, HOAc, HCl)" was added.
  • 11/7/95 - Nucleobond AX®, NucleoTrap®, NucleoTraPCR®, NucleoSpin®, NucleoSpin Extract®, and NucleoSpin Blood Extract® part numbers were changed from MN 730... to MN 740...
  • 8/15/95 - Nucleobond AX® DNA sequencing protocols added.
  • 8/3/95 - Protein & Peptide HPLC Columns technical information is now on-line.
  • 7/25/95 - The NucleoTrap®, NucleoTraPCR®, NucleoSpin®, and NucleoSpin Extract® properties and applications guides were added.
  • 7/25/95 - The Nucleobond AX® properties and applications guide was added.
  • 6/14/95 - The Vendor page was added.
  • 5/27/95 - The molecular biology WWW resource page was added.
  • 5/26/95 - Literature request and feedback forms were implemented.
  • 4/26/95 - The Nest Group, Inc. goes on-line with the opening of this Web site.

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    Last Updated: 03/12/08