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Merck SeQuant Newsletter - December 2008
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Our aim is to present interesting and valuable news to our readers, and we intend to highlight methods and strategies for HILIC separation of polar and hydrophilic compounds, such as peptides, metabolites, ions etc.

This edition - December 2008, focus on two new HILIC applications combined with different detection principles, Mass Spectromety (MS) and Evaporative light Scattering (ELSD).

Merck SeQuant develop and manufacture innovative products for separation and purification. We take pride in delivering high quality products and advanced technical support to customers world wide.


Dr Patrik Appelblad
Dr Tobias Jonsson


HILIC for Bioanalysis
Determination of Methylmalonic acid (MMA) in serum, plasma and urine is performed to monitor cobalamin (vitamin B12) deficiency. The assay is a functional marker of intracellular coalbumin status and is used for both diagnostic purposes as well as for monitoring treatment response. The assay requires simple protein precipitation and succinic acid does not interfere.

HILIC with ELSD
HILIC combined with evaporative light scattering detection (ELSD) is an excellent alternative for development of fast, sensitive, yet inexpensive methods for a wide range of solutes. Here it is illustrated by a separation of choline and inositol.


Merck SeQuant AB | Box 7956 | S-90719 Umeî | Sweden | Tel: +46-90-154880 | info@sequant.com