Chaotrope System Trouble Shooting:
Subsequent enzymatic reactions are inhibited
Subsequent enzymatic reactions are inhibited:
- Ethanol was not removed quantitatively. Residual ethanol can inhibit PCR*, LCR, and other enzymatic reactions.
- NucleoTrap particles were not removed quantitatively.
Centrifuge the eluate again and transfer the supernatant to a clean tube.
- Buffer NT2 was not removed quantitatively. Residual salt can inhibit enzymatic reactions.
* The polymerase chain reaction (PCR) process is covered by U.S. Patent No.
4,683,105 and 4,683,202 issued to Hoffman-La Roche. Patents pending in other
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