Fractionation of a purified tumor cell extract
Peptides ranging from 100fmol to 1pmol are collected in 40sec intervals during a 70min gradient from 0 - 60% MeCN with HFBA modifier.
HAISIL 300C18 5µm 40x2.1mm Part # H HF-0421-W185
 

Reanalysis of a fraction from the chromatogram in  Figure 1.
70min gradient from 0 - 60% MeCN with TFA modifier. Tic marks indicate 40sec fraction collection.
HAISIL 300 C18 5µm 40x2.1mm  P/N: H  HK-0421-W185

Higgins Analytical is grateful to Mark Ross and Argonex, Inc., Charlottesville, VA for permission to us to share this interesting work.