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Trap Columns Trap Columns

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Volume Dependent Results for Retention with Partition Column Chemistries:

Desorption increases with an excess of sample loading volume and/or wash volume. The maximum loading volume is one chromatographic void volume (ca. 60% of the bed volume) less than the k' of the analyte of interest. Increased loading can be obtained by either selecting a more retentive solvent condition or from a more retentive column chemistry such as TARGA C18, which allows loading in 100% water and which could even retain (for a few column volumes) underivatized simple sugars.

TARGA C18 equilibrates up to 10x faster than other RPC columns for shorter LC-MS cycle times which saves instrument costs.

Volume overload effects with SPE Tips

Effect of excessive loading and wash volumes on Trap Columns
Data, courtesy of Brian Hampton, University of Maryland, generated from a trapping experiment on a 0.3 x 30mm, TARGA C18, capillary trap. Various volumes of sample in 0.1% formic acid loaded onto a trap column equilibrated with 0.1% formic acid.


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Last Updated: 12/02/16