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Hydrophilic Interaction Chromatography (HILIC): Gentamycin (aminoglycosides) Antibiotics LC-MS/MS

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HILIC of Gentamycin (aminoglycosides) Antibiotics With LC-MS/MS Detection.

HILIC of aminoglycosides

McGrane et al., EuroResidue IV (May 2000), , Pg. 765 (van Ginkel & Ruiter, eds.), Bilthoven, The Netherlands.).

Gentamycin (aminoglycosides) Antibiotics Figure 5.1.8: (McGrane et al., EuroResidue IV (May 2000), Pg. 765 (van Ginkel & Ruiter, eds.), Bilthoven, The Netherlands.)
Chromatogram showing the separation achieved for aminoglycosides
HPLC Conditions ;    Column : PolyHYDROXYETHYL™ Aspartamide, 200 x 4.6 mm, 5 µm, 1000Å.
Mobile phase gradient ;    80 % acetonitrile, 20 % 250 mM NH4Ac, pH 4  at t=0,
       @ 1.0 ml min-1,    25 % acetonitrile, 75 % 250 mM NH4Ac, pH 4 at t=5 min.
25 % acetonitrile, 75 % 250 mM NH4Ac, pH 4 at t=15 min.
80 % acetonitrile, 20 % 250 mM NH4Ac, pH 4 at=18 min.,
Injection volume ;        50 µl

 

Poster HILIC of aminoglycosides

Poster: Use of 1000Å PolyHYDROXYETHYL™ Aspartamide for these very polar molecules reduced the amount of salt necessary for elution. Chromatogram showing the separation achieved for aminoglycosides

 

How HILIC works

Other HILIC References
 

PolyHYDROXYETHYL Part Numbers and Prices.


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Last Updated: 12/02/16