Hydrophilic Interaction Chromatography (HILIC): Gentamycin (aminoglycosides) Antibiotics LC-MS/MS
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HILIC of Gentamycin (aminoglycosides) Antibiotics With LC-MS/MS Detection.
McGrane et al., EuroResidue IV (May 2000), , Pg. 765 (van Ginkel & Ruiter, eds.), Bilthoven, The Netherlands.).
Gentamycin (aminoglycosides) Antibiotics
Figure 5.1.8: (McGrane et al., EuroResidue IV (May 2000), Pg. 765 (van
Ginkel & Ruiter, eds.), Bilthoven, The Netherlands.)
Chromatogram showing the separation achieved for aminoglycosides
HPLC Conditions ; Column : PolyHYDROXYETHYL™ Aspartamide,
200 x 4.6 mm, 5 µm, 1000Å.
Mobile phase gradient ; 80 % acetonitrile, 20 % 250
mM NH4Ac, pH 4 at t=0,
@ 1.0 ml min-1,
25 % acetonitrile, 75 % 250 mM NH4Ac, pH 4 at t=5 min.
25 % acetonitrile, 75 % 250 mM NH4Ac, pH 4 at t=15 min.
80 % acetonitrile, 20 % 250 mM NH4Ac, pH 4 at=18 min.,
Injection volume ; 50 µl
Poster: Use of 1000Å PolyHYDROXYETHYL™ Aspartamide for these very polar molecules reduced the amount of salt necessary for elution.
Chromatogram showing the separation achieved for aminoglycosides
How HILIC works
Other HILIC References
PolyHYDROXYETHYL
Part Numbers and Prices.
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Last Updated: 08/20/21