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PolyLC Ion Exchange Column Care & Cleaning

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General Information | Conditioning | Storage | Cleaning

General Information
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Conditioning
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Storage
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Notes:
In the refrigerator, microbial growth is minimal even if stored in 100% water. Based on our experience, it is possible to store it in ACN/water=50/50, or perhaps 75/25, without having to perform conditioning again. If you're going to use the column within a day or two, then there's no need to bother with anything but water.

Generally, a column will fail from something other than microbial growth, especially considering that mobile phase conditions with SCX (ion exchange) usually aren't conducive to microbial growth (i.e., some ACN and pH @ 3.0). However, if running ammonium acetate gradients at pH 5.5 without organic, then one has an excellent growth medium for microbes so we recommend flushing with water prior to storage.

If you flush the column with 100% ACN (or any other organic solvent) for storage, then condition the column with high salt when you take it out of storage, as if it were new.



Cleaning
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Last Updated: 12/02/16