DeOxy RiboNucleoside Analysis Using HILIC Conditions with Volatile Buffers

Under HILIC conditions (high organic solvent concentrations with a polar bonded phase column) elution is in the reverse order from RPC. The more polar molecules elute later.

Volatile buffers allow direct LC-MS detection.

Conditions: 1ml/min, isocratic, 90% ACN w 10mM NH4OAc, pH 4.5, A 254,
Column: PolyHYDROXYETHYL A® (P/N: 204HY0501, 100Å, 5µm, 4.6mm x 100mm)
Sample: DeOxy RiboNucleoside mix, dT,dA,dC,dG
 

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