MiniSpin & BioPureSPN SPE Column

MiniSpin & BioPureSPN SPE Column BioPureSPN SPE Columns

	Sample Capacity	Elution Volume	Bed Void Volume
BioPureSPN Mini™ SPE	0.03-70µg	10-40µL	20µL
BioPureSPN MIDI™ SPE	0.05-170µg	25-75µL	50µL
BioPureSPN MACRO™ SPE	0.03-350µg	50-150µL	100µL
96-Well MINI BioPureSPN Plate	0.03-70µg	10-40µL	20µL
96-Well MIDI BioPureSPN Plate	0.05-170µg	25-75µL	50µL
96-Well MACRO BioPureSPN Plate	0.03-350µg	50-150µL	100µL

ConSep™ mAb Conc & Buffer Exchg		115 --> 20µL
HisSep™ Histidine Rmvl Col	10-50µg	20-50µL
*PAGEEraser™ microFilter Columns**		5-200µL	0.1µl

Harvard Apparatus's MiniSpin columns have been obsoleted and are NO LONGER AVAILABLE. We are encouraging customers to switch to our new configuration for our RPC desalting MiniSpin columns, BioPureSPN, for additional sample capacity and reproducibility.

The BioPureSPN PROTO C18 MACRO (HMM S18V) contain the same wide pore C18 that is in the MACROspin C18 (SMM SS18V) columns (100mg vs. 89mg). The BioPureSPN PROTO C18 MIDI (HEM S18V) contain the same wide pore C18 that is in the MicroSpin C18 (SEM SS18V) columns (50mg vs. 38mg). The BioPureSPN PROTO C18 Mini (HUM S18V) contain the same wide pore C18 that is in the UltraMicroSpin C18 (SUM SS18V) columns (20mg vs. 18mg). The FastEq BioPureSPN C18 MACRO (HMM S18R) is a water wettable chemistry which equilibrates 10x faster than other C18's. It is the same TARGA C18 chemistry as is in the SMM SS18R columns, and is designed for impatient researchers or very polar molecules which need to be loaded in 100% water onto a column fully equilibrated at 100% water.

The extra packing provides greater assurance of binding from higher volume loads, but doesn't impose a volume penalty for elution since one spins these "dry." One can use exactly the same protocol currently used, with the assurance that this new package design will provide additional protection for one's samples. They are color coded by chemistry for convenience and are storable. Packaging options are at BioPureSPN.shtml.

Additionally, specific application materials like HILIC for detergent removal, HIL-SCX for post ICAT sample prep, ERLIC-WAX Phosphopeptide Enrichment are available from PolyLC® Inc., and TARGA® C18 for desalting of phosphopeptides and carbohydrates using 100% water loading conditions from Higgins Analytical® Inc.. Empty columns are available so your proprietary packings materials can be used in this format.

PAGE*Eraser™ microFilter Spin Columns remove particulates from samples from in-gel digests to prolong capillary LC-MS column life. Care must be taken to limit the number of cycles of sample solvent loading, since elution of sample can occur when more than seven bed volumes are employed for loading and wash. Additionally, the rate of solvent loading and elution will affect the ability of one's sample to diffuse onto or off of the bed. For these reasons, we prefer the use of BioPureSPE columns rather than Tips.

Detergent Removal: Use HILIC BioPureSPN columns for complete detergent removal.

Histidine Removal: Use HisSep™ Spin columns from ID Biologics replace membrane techniques for complete histidine removal from antibody formulations. HisSep™ columns increase the accuracy of assays by removing the interference of histidine in amino acid or icIEF electrophoretic assays.

Increased Accuracy Histidine Removal
Increased Accuracy of Antibody Assays After Histidine Removal

Volume Dependent Results for Salt Removal by SEC:

Bed
Void Volume Loading
Volume Desalting
Efficiency

96-Well MacroSpin Plate 95µL 125µL 100%

95µL 250µL >99%

95µL 400µL 78%

Volume Dependent Results for Retention with Partition Column Chemistries:

Desorption increases with an excess of sample loading volume and/or wash volume. The maximum loading volume is one chromatographic void volume (ca. 60% of the bed volume) less than the k' of the analyte of interest. Increased loading can be obtained by either selecting a more retentive solvent condition or from a more retentive column chemistry such as TARGA C18, which allows loading in 100% water and which could even retain (for a few column volumes) underivatized simple sugars.

Volume overload effects with SPE Tips
Effect of excessive loading and wash volumes on SPE Tips or Trap Columns
Data, courtesy of Brian Hampton, University of Maryland, generated from a trapping experiment on a 0.3 x 30mm, TARGA C18, capillary trap. Various volumes of sample in 0.1% formic acid loaded onto a trap column equilibrated with 0.1% formic acid.

Six-Step Method Development for Robust Solid Phase Extraction Results.

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Last Updated: 04/02/25

	Bed Void Volume	Loading Volume	Desalting Efficiency
96-Well MacroSpin Plate	95µL	125µL	100%
	95µL	250µL	>99%
	95µL	400µL	78%