Conventional SPE Cartridge Operating Instructions

HILICON® iSPE® HILIC SPE Cartridges

BioPureSPE™ TARGA® Fast Equilibrating, water wettable RPC.
BioPureSPE™ PROTO™ peptide protein RPC
TARGA® & PROTO™ are trademarks of Higgins Analytical, Inc.

Spin Column Operating Instructions

ConSep™ Antibody Concentration and Desalting Columns (115µL --> 20µL without membrane concentration or spinning).
HisSep™ Histidine Removal from Antibody Formulations


Glycan SPE
Activated Carbon: BioPureSPN Graphite for Glycan Clean-up. For additional details see Free Patent for Glycan Clean-up.
BioPureSPN™ MACRO HILIC
35-350µg capacity (ERLIC) HMM HIL, PolyHYDROXYETHYL A™ HILIC price list

BioPureSPN™ MIDI HILIC
17-170µg capacity (ERLIC) HEM HIL, PolyHYDROXYETHYL A™ HILIC price list

BioPureSPN™ MINI HILIC
7-70µg capacity (ERLIC) HUM HIL, PolyHYDROXYETHYL A™ HILIC price list

HILIC (...HIL, PolyLC®): Hydrophilic Interaction and Detergent Removal
HILIC cleanup of N-labelled glycans NEB Rapid PNGase F from 2AB, or procainamide reagent, NaCNBH4, DMSO & acetic acid labelling solution (with thanks to Paula Magnelli, New England Biolabs).
Unbonded Silica normal phase HILIC



Desalting
BioPureSPN™ MACRO RPC
Color Coded, Storable Desalting Columns, 35-350µg capacity (protein C4 [yellow] HMM S04V, PROTO™ 300 C4; peptide C18 [green] HMM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HMM S18R) price list

BioPureSPN™ MIDI RPC
Color Coded, Storable Desalting Columns, 17-170µg capacity (protein C4 [yellow] HEM S04V, PROTO™ 300 C4; peptide C18 [green] HEM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HEM S18R) price list

BioPureSPN™ Mini RPC
Color Coded, Storable Desalting Columns, 7-70µg capacity (protein C4 [yellow] HUM S04V, PROTO™ 300 C4; peptide C18 [green] HUM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HUM S18R) price list

MiniSpin™ RPC (...SS04V & ...SS18V, PROTO™ 300): Reversed phase peptide protein desalting
MiniSpin RP-sm (...SS18R, TARGA® C18): Polar molecule, hydrophobic desalting or enrichment from 100% water

BioPureSPN™ MACRO SEC and BioPureSPE™ SEC-60 instructions
Pre-Swelled, 20% MeOH preservative, SEC Desalting Columns, 10-100µL capacity (for protein desalting) price list
POLYHYROXYETHYL A™ SEC-60 Fractionating 96-Well Plate, 10-30µL loading price list

MiniSpin SEC (G10-G100, P2 and P6) dry resins (MACROspin recommended for capacity).

Phosphopeptide Desalting / Enrichment
Activated Carbon: BioPureSPN Graphite for phosphopeptide clean-up from salts and less polar peptides.
ERLIC-WAX (...HIL-DE, PolyLC): Phosphopeptide Enrichment (ASMS poster)
BioPureSPN™ MACRO HIL-WAX
35-350µg capacity (ERLIC) 0.2-1mg IEX; HMM HIL-WAX, PolyWAX LP™ WAX price list

BioPureSPN™ MIDI HIL-WAX
17-170µg capacity (ERLIC) 100-500µg IEX; HEM HIL-WAX, PolyWAX LP™ WAX price list

BioPureSPN™ MINI HIL-WAX
7-70µg capacity (ERLIC) 40-200µg IEX; HUM HIL-WAX, PolyWAX LP™ WAX price list

IMAC Phosphopeptide Enrichment (See also: S. Loroch et al., Biochimica et Biophysica Acta 1854 (2015) 460–468 Multidimensional ERLIC for ... phosphoproteome)
SCX (...HIL-SCX, PolyLC): Strong Cation Exchange: post ITRAC® & ICAT® clean-up, or subtractive phosphopeptide enrichment.
Peptide Protein IEX or ERLIC:
ERLIC-WAX (...HIL-DE, PolyLC): Phosphopeptide Enrichment (ASMS poster)
BioPureSPN™ MACRO HIL-WAX
35-350µg capacity (ERLIC) 0.2-1mg IEX; HMM HIL-WAX, PolyWAX LP™ WAX price list

BioPureSPN™ MIDI HIL-WAX
17-170µg capacity (ERLIC) 100-500µg IEX; HEM HIL-WAX, PolyWAX LP™ WAX price list

BioPureSPN™ MINI HIL-WAX
7-70µg capacity (ERLIC) 40-200µg IEX; HUM HIL-WAX, PolyWAX LP™ WAX price list

IMAC Phosphopeptide Enrichment: no longer available (See also: S. Loroch et al., Biochimica et Biophysica Acta 1854 (2015) 460–468 Multidimensional ERLIC for ... phosphoproteome)
SCX (...HIL-SCX, PolyLC): Strong Cation Exchange: post ITRAC® & ICAT® clean-up, or subtractive phosphopeptide enrichment. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.
BioPureSPN™ MACRO HIL-SCX
35-350µg capacity (ERLIC) 0.2-1mg IEX; HMM HIL-SCX, PolySULFOETHYL A™ SCX price list

BioPureSPN™ MIDI HIL-SCX
17-170µg capacity (ERLIC) 100-500µg IEX; HEM HIL-SCX, PolySULFOETHYL A™ SCX price list

BioPureSPN™ MINI HIL-SCX
7-70µg capacity (ERLIC) 40-200µg IEX; HUM HIL-SCX, PolySULFOETHYL A™ SCX price list

WAX Weak Anion Exchange (...HIL-DE, PolyLC) organic solvent compatible for ERLIC. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.
BioPureSPN™ MACRO HIL-WAX
35-350µg capacity (ERLIC) 0.2-1mg IEX; HMM HIL-WAX, PolyWAX LP™ WAX price list

BioPureSPN™ MIDI HIL-WAX
17-170µg capacity (ERLIC) 100-500µg IEX; HEM HIL-WAX, PolyWAX LP™ WAX price list

BioPureSPN™ MINI HIL-WAX
7-70µg capacity (ERLIC) 40-200µg IEX; HUM HIL-WAX, PolyWAX LP™ WAX price list

WCX Weak Cation Exchange (...HIL-WCX, PolyLC) organic solvent compatible. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.
BioPureSPN™ MACRO HIL-WCX
35-350µg capacity (ERLIC) 0.2-1mg IEX; HMM HIL-WCX, PolyCAT A™ WCX price list

BioPureSPN™ MIDI HIL-WCX
17-170µg capacity (ERLIC) 100-500µg IEX; HEM HIL-WCX, PolyCAT A™ WCX price list

BioPureSPN™ MINI HIL-WCX
7-70µg capacity (ERLIC) 40-200µg IEX; HUM HIL-WCX, PolyCAT A™ WCX price list

Protein IEX:
SAX Strong Anion Exchange (Sepharose® Q: limited to aqueous mobile phases only)
SCX Strong Cation Exchange (Sepharose® S: limited to aqueous mobile phases only)
WAX Weak Anion Exchange (Sepharose® DEAE: limited to aqueouss mobile phases only)
WCX Weak Cation Exchange (Sepharose® CM: limited to aqueous mobile phases only)

Overview & Prices for All Chemistries and Configurations

Spin-Con microDialysis Ultra-filtration for 1-5µl samples

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