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Storable, Semi-Reusable, MiniSpin SPE Columns

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Table of Contents

Applications:

Desalting Columns

Dialysis

Dye Removal

Electrophoresis

e-HILIC

Glycomics

HIC

HILIC

Ion Chromatography

Organic Acids

Peptides & Proteins

Process Columns

Small Molecules

Company

Archives

Related Links:

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Micro-Sample Techniques

(Activated Carbon, HILIC, ERLIC, RPC & SEC) microSPE columns & 96-Well plates

Conventional SPE Cartridge Operating Instructions

    SPE Columns
    HILICON® iSPE® HILIC SPE Cartridges

    BioPureSPE™ TARGA® Fast Equilibrating, water wettable RPC.
    BioPureSPE™ PROTO™ peptide protein RPC
    TARGA® & PROTO™ are trademarks of Higgins Analytical, Inc.

Spin Column Operating Instructions

    Spin Columns
    ConSep™ Antibody Concentration and Desalting Columns (115µL --> 20µL without membrane concentration or spinning).
    HisSep™ Histidine Removal from Antibody Formulations


    Glycan SPE
    Activated Carbon: BioPureSPN Graphite for Glycan Clean-up. For additional details see Free Patent for Glycan Clean-up.
    BioPureSPN™ MIDI HILIC
      17-170µg capacity (ERLIC) HEM HIL, PolyHYDROXYETHYL A™ HILIC price list

    BioPureSPN™ MINI HILIC
      7-70µg capacity (ERLIC) HUM HIL, PolyHYDROXYETHYL A™ HILIC price list

    HILIC (...HIL, PolyLC®): Hydrophilic Interaction and Detergent Removal
    HILIC cleanup of N-labelled glycans NEB Rapid PNGase F from 2AB, or procainamide reagent, NaCNBH4, DMSO & acetic acid labelling solution (with thanks to Paula Magnelli, New England Biolabs).
    Unbonded Silica normal phase HILIC

    BioPureSPN MACRO Column Color Coded Spin Column

    Desalting
    BioPureSPN™ MACRO RPC
      Color Coded, Storable Desalting Columns, 35-350µg capacity (protein C4 [yellow] HMM S04V, PROTO™ 300 C4; peptide C18 [green] HMM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HMM S18R) price list

    BioPureSPN™ MIDI RPC
      Color Coded, Storable Desalting Columns, 17-170µg capacity (protein C4 [yellow] HEM S04V, PROTO™ 300 C4; peptide C18 [green] HEM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HEM S18R) price list

    BioPureSPN™ Mini RPC
      Color Coded, Storable Desalting Columns, 7-70µg capacity (protein C4 [yellow] HUM S04V, PROTO™ 300 C4; peptide C18 [green] HUM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HUM S18R) price list

    MiniSpin™ RPC (...SS04V & ...SS18V, PROTO™ 300): Reversed phase peptide protein desalting
    MiniSpin RP-sm (...SS18R, TARGA® C18): Polar molecule, hydrophobic desalting or enrichment from 100% water
    BioPureSPN™ MACRO SEC
      Pre-Swelled, Azide Stabilized, SEC Desalting Columns, 10-100µL capacity (for protein desalting) price list

    MiniSpin SEC (G10-G100, P2 and P6 resins) by size (MACROspin recommended for capacity).

    Phosphopeptide Desalting / Enrichment Peptide Protein IEX or ERLIC:
    Protein IEX:
      SAX Strong Anion Exchange (Sepharose® Q: limited to aqueous mobile phases only)
      SCX Strong Cation Exchange (Sepharose® S: limited to aqueous mobile phases only)
      WAX Weak Anion Exchange (Sepharose® DEAE: limited to aqueouss mobile phases only)
      WCX Weak Cation Exchange (Sepharose® CM: limited to aqueous mobile phases only)

Overview & Prices for All Chemistries and Configurations

    Spin-Con microDialysis Ultra-filtration for 1-5µl samples

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Sepharose®is a Trademark of Amersham Pharmacia Biotech

 


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For more information or to place an order contact:
The Nest Group, Inc.™   45 Valley Road   Southborough, MA   01772-1323   USA
Tel: 800-347-6378 or 1-508-481-6223   Fax: 1-508-485-5736
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Last Updated: 08/16/18