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MicroSPE Methods Index

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Micro-Sample Techniques (MiniSpin columns, 96-Well Spin plates, microDialysis Ultra-filtration)

Conventional SPE Cartridge Operating Instructions

    SPE Columns
    HILICON® iSPE® HILIC SPE Cartridges

    BioPureSPE™ TARGA® Fast Equilibrating, water wettable RPC.
    BioPureSPE™ PROTO™ peptide protein RPC
    TARGA® & PROTO™ are trademarks of Higgins Analytical, Inc.

Spin Column Operating Instructions

    Spin Columns
    ConSep™ Antibody Concentration and Desalting Columns (115µL --> 20µL without membrane concentration or spinning).
    HisSep™ Histidine Removal from Antibody Formulations

    Glycan SPE
    Active Charcoal for Glycan Clean-up. For additional details see Free Patent for Glycan Clean-up.
    HILIC (...HIL, PolyLC®): Hydrophilic Interaction and Detergent Removal
    HILIC cleanup of N-labelled glycans NEB Rapid PNGase F from 2AB, or procainamide reagent, NaCNBH4, DMSO & acetic acid labelling solution (with thanks to Paula Magnelli, New England Biolabs).
    Unbonded Silica normal phase HILIC

    BioPureSPN MACRO Column Color Coded Spin Column

    Desalting
    Semi-Reusable BioPureSPN™ MACRO , Color Coded, RPC Desalting Columns, 30-300g capacity (protein C4 [yellow] HMM S04V, PROTO™ 300 C4; peptide C18 [green] HMM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HMM S18R) price list
    Semi-Reusable BioPureSPN™ MIDI, Color Coded, RPC Desalting Columns, 6-60g capacity (protein C4 [yellow] HEM S04V, PROTO™ 300 C4; peptide C18 [green] HEM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HEM S18R) price list

    MiniSpin RPC (...SS04V & ...SS18V, PROTO™ 300): Reversed phase peptide protein desalting
    MiniSpin RP-sm (...SS18R, TARGA® C18): Polar molecule, hydrophobic desalting or enrichment
    MiniSpin SEC (G10-G100, P2 and P6 resins) by size (MACROspin recommended for capacity).

    Peptide Protein IEX or ERLIC:
    ERLIC-WAX (...HIL-DE, PolyLC): Phosphopeptide Enrichment (ASMS poster)
    IMAC Phosphopeptide Enrichment (See also: S. Loroch et al., Biochimica et Biophysica Acta 1854 (2015) 460468 Multidimensional ERLIC for ... phosphoproteome)
    SCX (...HIL-SCX, PolyLC): Strong Cation Exchange: post ITRAC® & ICAT® clean-up, or subtractive phosphopeptide enrichment. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.
    WAX Weak Anion Exchange (...HIL-DE, PolyLC) organic solvent compatible for ERLIC. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.
    WCX Weak Cation Exchange (...HIL-WCX, PolyLC) organic solvent compatible. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.

    Protein IEX:
    SAX Strong Anion Exchange (Sepharose® Q: limited to aqueous mobile phases only)
    SCX Strong Cation Exchange (Sepharose® S: limited to aqueous mobile phases only)
    WAX Weak Anion Exchange (Sepharose® DEAE: limited to aqueouss mobile phases only)
    WCX Weak Cation Exchange (Sepharose® CM: limited to aqueous mobile phases only)
MiniSpin pdf Overview of Chemistries and Configurations

Spin-Con microDialysis Ultra-filtration for 1-5µl samples

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Sepharose®is a Trademark of Amersham Pharmacia Biotech

 


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Last Updated: 06/20/17