Micro-Sample Techniques (MiniSpin columns, 96-Well Spin plates, microDialysis Ultra-filtration)
Conventional SPE Cartridge Operating Instructions
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HILICON® iSPE® HILIC SPE Cartridges
BioPureSPE™ TARGA® Fast Equilibrating, water wettable RPC.
BioPureSPE™ PROTO™ peptide protein RPC
Spin Column Operating Instructions
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ConSep™ Antibody Concentration and Desalting Columns (115µL --> 20µL without membrane concentration or spinning).
HisSep™ Histidine Removal from Antibody Formulations
Glycan SPE
Active Charcoal for Glycan Clean-up. For additional details see Free Patent for Glycan Clean-up.
HILIC (...HIL, PolyLC®): Hydrophilic Interaction and Detergent Removal
HILIC cleanup of N-labelled glycans NEB Rapid PNGase F from 2AB, or procainamide reagent, NaCNBH4, DMSO & acetic acid labelling solution (with thanks to Paula Magnelli, New England Biolabs).
Unbonded Silica normal phase HILIC
Desalting
Semi-Reusable BioPureSPN™ MACRO , Color Coded, RPC Desalting Columns, 30-300µg capacity (protein C4 [yellow] HMM S04V, PROTO™ 300 C4; peptide C18 [green] HMM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HMM S18R) price list
Semi-Reusable BioPureSPN™ MIDI, Color Coded, RPC Desalting Columns, 6-60µg capacity (protein C4 [yellow] HEM S04V, PROTO™ 300 C4; peptide C18 [green] HEM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HEM S18R) price list
MiniSpin RPC (...SS04V & ...SS18V, PROTO™ 300): Reversed phase peptide protein desalting
MiniSpin RP-sm (...SS18R, TARGA® C18): Polar molecule, hydrophobic desalting or enrichment
MiniSpin SEC (G10-G100, P2 and P6 resins) by size (MACROspin recommended for capacity).
Peptide Protein IEX or ERLIC:
ERLIC-WAX (...HIL-DE, PolyLC): Phosphopeptide Enrichment (ASMS poster)
IMAC Phosphopeptide Enrichment (See also: S. Loroch et al., Biochimica et Biophysica Acta 1854 (2015) 460–468 Multidimensional ERLIC for ... phosphoproteome)
SCX (...HIL-SCX, PolyLC): Strong Cation Exchange: post ITRAC® & ICAT® clean-up, or subtractive phosphopeptide enrichment. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.
WAX Weak Anion Exchange (...HIL-DE, PolyLC) organic solvent compatible for ERLIC. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.
WCX Weak Cation Exchange (...HIL-WCX, PolyLC) organic solvent compatible. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.
Protein IEX:
SAX Strong Anion Exchange (Sepharose® Q: limited to aqueous mobile phases only)
SCX Strong Cation Exchange (Sepharose® S: limited to aqueous mobile phases only)
WAX Weak Anion Exchange (Sepharose® DEAE: limited to aqueouss mobile phases only)
WCX Weak Cation Exchange (Sepharose® CM: limited to aqueous mobile phases only)
Spin-Con microDialysis Ultra-filtration for 1-5µl samples
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