Nest Group Logo








Volatile Buffer pH Ranges for LCMS & ELSD Detectors Volatile Buffer pH Range Table

For your convenience, we accept Mastercard, VISA, and American Express credit cards.


| Home | IdeaBook | Orders | Price/Applic | Price/Vendor | Protocols|
 

Volatile Buffers for LCMS & ELSD Detectors

Buffer Preparation Calculator (University of Liverpool). Choose the buffer species you want to use. Enter parameters for volume, pH, and concentration of buffer species, with an option to modify the ionic strength by addition of neutral salt. Finally, enter the temperature at which you'll use the buffer, and the temperature at which you'll make it up. All in all and excellent tool you'll find useful.

Reagent
Formula
Weight
Density
Approx.
Strength
Molarity
(M)
Normality
(N)
Useful pH Range
lower
higher
Acetic Acid1 (CH3COOH)
60.052
1.05
99.8%
17.4
17.4
4.3 - 5.3
Ammonia : Acetic Acid (NH3 : CH3COOH)
77.083
4.3 - 5.3
8.8 - 9.8
Ammonia : Formic Acid (NH3 : HCOOH)
63.057
3.3 - 4.3
8.8 - 9.8
Ammonium Acetate (CH3CONH4)
77.0825
1.17
3.8 - 5.8
8.2 - 10.2
Ammonium Bicarbonate (NH4HCO3)
79.056
1.59
5.9 - 6.9
8.8 - 9.8
Ammonium Carbonate ((NH4)2CO3)
96.09
1.50
5.9 - 6.9
8.8 - 9.8
Ammonium Formate (HCONH4)
63.0559
1.28
2.8 - 4.8
8.2 - 10.2
Formic Acid1 (HCOOH)
46.026
1.13
90%
23.6
23.6
3.3 - 4.3
Pyridine : Formic Acid (C6H6NO2)
125.048
3.3 - 4.3
4.8 -5.8
Trimethylamine : Formic Acid (C3H9N : HCOOH)
105.14
3.3 - 4.3
9.3 - 10.3
Trimethylamine : Acetic Acid (C3H9N : CH3COOH)
114.49
4.3 -5.3
9.3 - 10.3
1-Methylpiperidene (C6H13N)
99.17
1.71
> 98%
10.0 - 12.0
Ammonium Hydroxide
(NH4OH)
35.046
0.90
 56.6%4
14.5
14.5
(1) Formic acid is slightly stronger and more volatile than Acetic acid. Neither Acetic Acid nor Formic Acid are buffers alone, which results in wider analyte peaks.
(2) While Trifluoroacetic acid (TFA) is volatile, it is not a buffer, nor recommended for LC/MS applications since it can cause matrix effect baseline contamination and can be hard to remove from the MS source.

Copyright© 1995-2017 The Nest Group, Inc.™ All rights reserved (established 1984)

For more information or to place an order contact:
The Nest Group, Inc.™ · 45 Valley Road · Southborough, MA  01772-1323
Tel: 800-347-6378 or 508-481-6223 · Fax: 508-485-5736
For your convenience, we accept Mastercard, VISA, and American Express credit cards.


| Home | IdeaBook | Orders | Price/Applic | Price/Vendor | Protocols|

If you have problems or comments concerning our WWW service, please send an e-mail to Amos Heckendorf, webmaster.

 
About Us | Privacy Policy | Trademarks | Contact Us

Last Updated: 12/02/16