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HILICON® iHILIC® & iSPE® Columns and SPE Cartridges HILICON® iHILIC® & iSPE® Columns and SPE Cartridges

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Analytical (2.1mm - 4.6mmID) iHILIC® Fusion and Fusion(+) columns used in an ERLIC vs. HILIC mode. Solid Phase Extraction Cartridges, iSPE® HILIC (~Fusion(+) silica)

Modulated Charge HydroxyEthyl Amide iHILIC® Fusion columns

Modulated Charge HydroxyEthyl Amide iHILIC Fusion columns

Effect of Buffer Strength on iHILIC® Fusion columns

Modulated Charge HydroxyEthyl Amide iHILIC Fusion columns

Applications:

  • Amino Acids

    Underivatized Amino Acids from plant extracts

    Direct Analysis of Amino Acids by HILIC–ESI-MS. Alexander Schriewer ¹, Katharina Johanna Heilen¹, Heiko Hayen¹, and Wen Jiang², LCGC, THE APPLICATION NOTEBOOK – July 2017. ¹Institute of Inorganic and Analytical Chemistry, University of Münster, Münster, Germany, ²HILICON AB

    Underivatized Amino Acids Metabolomics LC/MS

  • Artificial Sweetners
    SucraloseSucralose AcesulfameAcesulfame K SaccharinSaccharin NeotameNeotame
    NeohesperidinNeohesperidin CyclamateNa Cyclamate AspartameAspartame

    Artifical Sweetners on iHILIC® Fusion (+) columns
    iHILIC® Fusion(+), Column: 2.1 x 150mm, 100Å, 3.5µm, 0.3 mL/min.
    Gradient elution: A) acetonitrile; B) 10mM ammonium formate, pH = 3.5; gradient elution from (95/5) A/B to (84/16) A/B in 8.5 min.
    Column temperature: 40 °C
    Injection volume: 5 µL
    Data Courtesy of University of Münster

  • Glucuronides and Glucosides
    UDP-Glucose Structure
    iHILIC® Fusion(+), 2.1 x 100mm, 100Å, 3.5µm, 0.4mL/min.
    Gradient 90:10 to 30:70; ACN:10mM, pH 3.4, ammonium acetate w/0.1% FA (v/v)), 10µL injection.
    Data Courtesy of Mike Karb, P&G.

  • UDP-Glucose
    UDP-Glucose Structure UDP-Glucose on iHILIC® Fusion column
    iHILIC® Fusion, 2.1 x 100mm, 100Å, 3.5µm, 0.2mL/min.
    Gradient 80:20 to 60:40; ACN:100mM, pH 5.8, ammonium acetate (v/v))
    Data Courtesy of Narek Darabedian in Dr. Matt Pratt's lab, USC.
    Publication: "Metabolic chemical reporters of glycans exhibit cell-type selective metabolism and glycoprotein labeling." Anna R. Bhatt, et al., ChemBioChem 10.1002/cbic.201700020


  • cGAMP
    cGAMP on iHILIC® Fusion column
    iHILIC® Fusion, 2.1 x 100mm, 100Å, 3.5µm, 0.2mL/min.
    Gradient 90:10 to 50:50; ACN:50mM, pH 5, ammonium acetate (v/v))
    Data Courtesy of Dr. Michelle Dubuke in Dr. Scott Shaffer's lab, UMass Medical Center.



  • Mono-, Di- & Tri-phosphate Nucleotides
    Nucleotides on iHILIC® Fusion columns
    iHILIC® Fusion, 2.1 x 100mm, 100Å, 3.5µm, 0.2mL/min.
    Nucleotides Separate at a lower buffer concentration on iHILIC® Fusion at pH 5.8, 70:30; ACN:100mM, pH 5.8, ammonium acetate (v/v) than at pH 4.5, 70:30; ACN:100mM, on ZIC® cHILIC due to less titrating to lower the pH.


  • Enhanced sensitivity of phosphates by MS at high pH:
    nTP on iHILIC® Fusion(P) columns
    iHILIC® Fusion(P), 4.6 x 150mm, 200Å, 5µm.
    Separation of nTP's at pH 9.3 with a volatile buffer: Gradient. Buffer A: 80:20 (v/v) Acetonitrile, 100mM ammonium bicarbonate and Buffer B: 30mM ammonium bicarbonate pH 9.3.

    Metabolomics (ATP/ADP ratios, acetyl-CoA). "Breast Cancer-Derived Lung Metastases Show Increased Pyruvate Carboxylase-Dependent Anaplerosis," Christen et al., 2016, Cell Reports 17, 837–848 October 11, 2016 and supplemental information. MS in negative mode. Column iHilic Fusion(P). Solvent: ACN and pH=9.3, 10mM ammonium acetate.

  • Separation of 2'NADP from 3'NADP at high pH:
    NADP on iHILIC® Fusion(P) columns NADP on iHILIC® Fusion(P) columns
    iHILIC® Fusion(P), 4.6 x 150mm, 200Å, 5µm.
    Separation of NADP's at pH 9.3 with a volatile buffer: Gradient. Buffer A: 80:20 (v/v) Acetonitrile, 20mM ammonium bicarbonate and Buffer B: 20mM ammonium bicarbonate pH 9.3.
    Unpublished data courtesy of Corey D. Broeckling, Colorado State University.

  • Organic Acids:
    GHB on iHILIC® Fusion(P) columns GABA on iHILIC® Fusion(P) columns Succinic Acid on iHILIC® Fusion(P) columns
    iHILIC® Fusion(P) PEEK, 2.1 x 50mm, 5µm, 200Å, 5µm.
    Isocratic separation of γ-hydroxybutyric acid (GHB), γ-aminobutyric acid (GABA) & succinic acid with a volatile buffer: Buffer A: 90:10 (v/v) Acetonitrile, 20mM ammonium acetate.
    Unpublished data courtesy of Patrick Belanger, INSPQ - Centre de Toxicologie (CTQ). See also: Effects of pH and Buffer Strength, an Organic Acids Study.

  • Enhanced sensitivity of inorganic ion detection with ELSD when using the polymeric Fusion(P):
    Elimination of silica leaching increases sensitivity.
    ions on iHILIC® Fusion(P) columns
    iHILIC® Fusion(P), 4.6 x 150mm, 200Å, 5µm.
    Separation of ammonia and sulfate:
    Mobile phase: A) Acetonitrile; B) 25 mM ammonium acetate, pH 6.8 (salt solution without pH adjustment)
    Gradient program: 0 min: 85% A, 6 min: 65% A, 6-9 min: 65% A, 9-16 min: 85% A.
    Flow rate: 0.2 mL/min. Detector: NQAD
    Peaks: #2: Ammonium, #3: Sulfate.

    iHILIC® Fusion(P), out performs ZIC-pHILIC for analysis of inorganic ions
    ions on iHILIC® Fusion(P) columns
    iHILIC® Fusion(P), 4.6 x 150mm, 200Å, 5µm.
    Gradient: 85% ACN/15% 25 mM ammonium acetate to 60% ACN/40% 25 mM ammonium acetate.
    When using iHILIC® Fusion(P), thiosulfate is a sharp symmetrical peak, sulfate elutes after thiosulfate as a sharp symmetrical peak and sodium is more retained than on the ZIC®pHILIC column (shown above).
    Data courtesy of Paul Kostel, Eurofins Advantar Labs., Inc.

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    Last Updated: 08/01/17