Training
- Basics of Dialysis and Ion Exchange
Designing a purification protocol using ion exchange resins
"Basics of HPLC," an on-line book by Dr. Yuri Kazakevich and Dr. Harold McNair
"Buffer Solubility in Aqueous-Organic Solvents" Adam P. Schellinger and Peter W. Carr, LCGC, JUNE (2004) 22(6): 544-48. Useful for RPC and/or HILIC mobile phase composition selection.
The Importance of Contact Time for Either Desalting or Trapping of Polar Solutes on Trap Columns.
Measuring pH in aqueous/organic mixtures:
-
Use of the s(w)pH scale method. [Canals I, JA Portal, E Bosch, and M Roses
Retention of ionizable compounds on HPLC. 4. Mobile-phase pH measurement in methanol/water
Anal Chem, April 15, 2000; 72(8): 1802-9.]
- Three
procedures are evaluated:
- - measurement of the pH of the aqueous HPLC buffer before mixing it with
the organic modifier,
- measurement of the pH of the HPLC buffer after mixing it with the organic modifier using a pH electrode system calibrated with aqueous buffers, and
- measurement of the pH of the HPLC buffer after mixing it with the organic modifier but calibrating the electrode system with reference buffers prepared in the same mixed solvent used as mobile phase.
-
"Mobile-Phase Buffers, Part I The Interpretation of pH in Partially Aqueous Mobile Phases." A pH change occurs when organic solvent is present and how silica columns can be used at a higher pH.
"Mobile-Phase Buffers, Part II Buffer Selection and Capacity." Selecting buffers for use in partially aqueous mobile phases.
"Mobile-Phase Buffers, Part III Preparation of Buffers." The importance of using the weights-and-volumes format.
Retention Time Predictor Tool for ZIC-HILIC columns.
Sample Loading and Gradient Modification Data Tables for Column Dimension Changes.
HPLC Column Care
-
HIC for Proteins:
(ALKYL) Aspartamide™ Hydrophobic Intereaction Technical Sheet (Mfg. Instruction Sheet)
Trouble shooting HYDROCELL™ polymeric HIC columns.
Buffer Tables for HIC and IEX.
HILIC for Polar Differences in Molecules:
PolyHYDROXYETHYL™ Aspartamide Technical Sheet (Mfg. Instruction Sheet)
PolyGLYCOPLEX™ (for Complex Carbohydrates) Technical Sheet (Mfg. Instruction Sheet)
ZIC®-HILIC & ZIC®-pHILIC General Operational Parameters Before Getting Started:
- ZIC®-HILIC Columns Technical Sheet
ZIC®-pHILIC Polymeric Columns Technical Sheet
ZIC®-HILIC Capillary Columns Technical Sheet
Ion Exchange:
PolySULFOETHYL™ Aspartamide Strong Cation Exchange Technical Sheet (Mfg. Instruction Sheet)
PolyCAT A™ Weak Cation Exchange Technical Sheet (Mfg. Instruction Sheet)
PolyWAX LP™ Weak Anion Exchange, Technical Sheet (Mfg. Instruction Sheet)
PolyCAT A - PolyWAX LP, Mixed-Bed Ion Exchange Technical Sheet (Mfg. Instruction Sheet)
Trouble shooting HYDROCELL™ polymeric IEX columns.
Buffer Tables for IEX and HIC.
Reversed Phase:
General Column Care Information
Vydac Column Care
Vydac Comparison of the All-Guard™ Cartridges to the Vydac "...GD54" Guard Cartridges
Size Exclusion (SEC):
SEC Column PolyHYDROXYETHYL Aspartamide Technical Sheet (Mfg. Instruction Sheet)
Small Molecule and Combi-Chem SPE Separations
Cerex Filtration/Drying Manifolds & SPE Cartridges for Purificaton and Recovery
-
Cerex System-48 or
Cerex System-96 for positive pressure SPE filtration or drying
- View Demo Cerex System-48
View Demo Cerex System-96
Cerex SPE Cartridges and Plates for high performance (small particle) SPE. Small particle SPE means higher speed, higher loading, and smaller elution volume SPE.
UniFlash Planar Station™, four channel, fast TLC development chamber.
-
TLC to Flash: Optimizing Your TLC
TLC to Flash: Estimation of Load
TLC to Flash: Flash Solvent Selction
TLC to Flash: Gradient Techniques
MiniSpin and MicroTip micro-SPE & Desalting Kits:
- MiniSpin
columns and 96-Well
Spin Plates for microliter sample prep. Concentrate, desalt
or fractionate 2 - 400µl of chemical or biological materials
on over 20 RPC, IEX, and Polar micro-SPE chemistries or 2 - 150µl on SEC materials.
- UltraMicroTip Part Numbers and Prices
- MiniSpin & 96-Well Spin Part Numbers and Prices
Operating Instructions for HILIC, RPC, SCX, SEC, WAX, or WCX MiniSpin products.
Empty µ-Reactors™ with caps to remove solid reactants from micro quantities of samples. See: Xia B., et al. Anal Biochem. 2009 Apr 15;387(2):162-70. Epub 2009 Feb 10. Glycan reductive isotope labeling (GRIL) for quantitative glycomics.
Ultra-Micro PrepTip™ MS µ-SPE Tips:
- Pipette tips to concentrate, desalt or fractionate 1 - 10µL and 10 - 100µL of
biological materials.
- Ultra-Micro PrepTip Part Numbers and Prices
Ultra-Micro PrepTip™ RP clean-up for detergent or salt removal from protein digests. Useful for MS applications, due to lower back pressure and higher capacity than ZipTip® C18.
Small Molecule and Combi-Chem HPLC Separations
Higgins Analytical® HPLC columns
(Exceptionally Good Performance, A Wide Range of Configurations.) Our "Best Value" Product Line.-
Complete Catalog (3,408K pdf)
Catalogs of HAISIL® HPLC Columns (0.3 - 22mmID), or Short, Direct Connect, 2.1mmID Narrowbore (Sprite), 0.3-1.0mmID Microbore Guard/Trap (Piccolo), 0.075 - 0.15mmID Nano-Capillaries (Capellini), and/or Cartridge (HAIPEEK) Products:
-
(Type A Silica)
- HAISIL 100™ (small pore) products
HAISIL 300™ (wide pore) products
Kromasil® 100 small pore columns.
- Comparison of Kromasil to PHALANX for pharmaceutical method equivalency.
-
HAISIL RS, PROTO™ 200 C4 & C18 for Peptide applications.
- Comparison of PROTO™ 200 to Grace/Vydac® C18 Peptide Columns.
Comparison of PROTO 200 for Synthetic Peptides.
HAISIL TS, TARGA ® C8 & C18 for Polar Analytes & Proteomics.
HAISIL CS, CLIPEUS™ Silica, CN, Phenyl, C8, & C18 phases, excellent for Small Molecule applications.
-
HAISIL VS, HEAVY™ Ultra-high density coating for wide pH range applications.
HAISIL HL, High Load High density coating for Combi-Chem & Fast LC Analyses.
HAISIL PS, PHALANX™ Water-wettable, Ultra-high density coating for wide pH range applications.
-
PL-Gel® PLRP-S, 3µm, 100Å pores, for Small Molecule, high pH applications, such as negative ion mode LC/MS; or 300Å pores for Peptide applications.
PLRP-S, RP1K & RP4K, 5µm, with 1000Å or 4000Å pores for Protein & Antibody RPC.
PL-SAX, PL-SCX, PL-WAX & PL-WCX, 5µm, with 1000Å or 4000Å pores for Protein and Antibody ion exchange applications.
- Amino acids on HAISIL Columns:
-
TARGA (low organic, 100% water wettable RPC) for DABSYL or AccQ-Tag® derivatives
High Load for fast PTH analyses
HAISIL PTC (ABI 711-0204) for PTC analyses
HAISIL 100 for Pico-Tag® derivatives
-
Amines (without tailing), or for LC-MS/MS, "no TFA"
peptide separations: CLIPEUS with WOW! selectivity in C8, C18, CN or Phenyl phases that do not
require base deactivation.
Combi-Chem analyses of small molecules on Heavily Loaded HAISIL columns
PROTO™ 200 C4 & C18 Peptide Columns
-
Comparison of PROTEO™ C18 to Other Columns for Phosphopeptides.
-
Comparison of PROTO300™ C4 to Vydac C4 for proteins.
Comparison of PROTO™300 C4 to Vydac C8 and Zorbax® C8 for protein digests.
-
Comparison of TARGA to BetaBasic™ for Polar Peptides
HAISIL PEEK and HAIPEEK HPLC Cartridge Columns.
Higgins Columns Part Numbers and Prices
MACCEL™ HPLC Columns
-
Enhanced polar selectivity on MACCEL AQPS™, a water wettable (low organic RPC) phase, with great selectivity for
all polar molecules.
- Engelhard Test: Polar Selectivity of unbuffered acids and bases on AQPS phases.
Polar Selectivity of acids and bases on AQPS phases under acidic buffered, ACN conditions.
Optimum Conditions for acids and bases on AQPS phases: neutral buffered, MeOH conditions. - Compare selectivity on MACCEL Spheribond™ ODS1 and ODS2 to Waters™ Spherisorb® ODS1 and ODS2
- Spheribond™ ODS1: Selectivity is similar under a number of comparative conditions.
Spheribond™ ODS2: Selectivity is very close under a number of comparative conditions. - Polar molecule selectivity on MACCEL Hypersorb™ ODS is very close to Thermo™ Hypersil® ODS
- Hypersorb™ ODS: Selectivity under a number of comparative conditions is very similar.
- Maccel Analytical and Microbore HPLC columns
MACCEL Small Molecule HPLC Application Notes
MACCEL Part Numbers and Prices
Grace/Vydac® Small Molecule HPLC Columns
-
Denali® C18 (monomeric) Ultra High Peak Symmetry Small Molecule Columns.
Vydac Application Notes
Vydac Column Care
Part Numbers & Prices "238DE™ Series".
Complex Carbohydrate Separations
Glycan Reductive Isotope Labeling (GRIL)
- Empty µ-Reactors™
with caps remove solid reactants from micro quantities of samples. See: Xia B., et al. Anal Biochem. 2009 Apr 15;387(2):162-70. Epub 2009 Feb 10. Glycan reductive isotope labeling (GRIL) for quantitative glycomics.
Using HILIC's Electrostatic and Hydrogen Donor Mechanisms for Carbohydrate Separations.
- Complex Carbohydrate ZIC- HILIC References
ERLIC conditions for Sialylated or Sulfated Carbohydrates
IP-NPLC [eHILIC] for high mannose and sialylated glycopeptides. "Identification and Quantification of Glycoproteins Using Ion-Pairing Normal-Phase LC and MS," Wen Ding, Harald Nothaft, Christine M. Szymanski, and John Kelly, MCP Papers in Press, June 12, 2009.
PolyGLYCOPLEX™ for Complex Carbohydrates, Spec Sheet
PolyGLYCOPLEX Bibliographic references.
HPLC Columns for Protein & Peptide Analysis Applications:
Detergent Removal
Technical Sheet for HILIC microSPE Detergent Removal Cartridges or SDS Removal Guard columns.Peptide Retention Time Calculations
-
Oleg Krokhin's RT Calculator. New sequence-specific
correction factors for prediction of peptide retention in RP-HPLC: application to
protein identification by off-line HPLC-MALDI-MS. This version has been developed for the same set of ~2000
tryptic peptides and features new correction factors related to a peptide's propensity to form helical
structures. Correlation about 0.98 (R-squared value) was obtained for the training set of 2000 peptides
compared to 0.96 for version 2.
Higgins Analytical HILIC and RPC Peptide Columns
-
Complete Catalog (3,408K pdf)
HILIC HAISIL® 100 Silica Columns and Cartridge (HAIPEEK) Products.
Amino acids on HAISIL Columns:
-
TARGA (low organic, 100% water wettable RPC) for DABSYL or AccQ-Tag® derivatives
High Load for fast PTH analyses
HAISIL PTC (ABI 711-0204) for PTC analyses
HAISIL 100 for Pico-Tag® derivatives
PHALANX Water-wettable, Ultra-high density coating for a wider pH range for peptide applications.
Proteomics: Fast equilibration & 100% aqueous loading on TARGA, allows for an extremely wide polarity range for LC/MS/MS "no TFA" peptide separations.
-
Comparison of TARGA to BetaBasic™ for Polar Peptides
-
Comparison of PROTEO 200 to Grace/Vydac® Peptide Columns.
Comparison of PROTO 200 for Synthetic Peptides.
-
Comparison of PROTO300™ C4 to Vydac C4 for proteins.
Comparison of PROTO™300 C4 to Vydac C8 and Zorbax® C8 for protein digests.
"Wide pore" HAISIL® Reversed Phase (Type A Silica) HPLC Columns and Cartridge Products have been used for virus particles and protein, peptide separations.
Polymeric RPC and Ion Exchange (Capillary - < 2mmID column configurations.)
-
PL-Gel® PLRP-S, 3µm, 100Å pores, for Small Molecule, high pH applications, such as negative ion mode LC/MS; or 300Å pores for Peptide applications.
PLRP-S, RP1K & RP4K, 5µm, with 1000Å or 4000Å pores for Protein & Antibody RPC.
PL-SAX, PL-SCX, PL-WAX & PL-WCX, 5µm, with 1000Å or 4000Å pores for Protein and Antibody ion exchange applications.
Higgins Columns Part Numbers and Prices
PolyLC®
- Ion Exchange columns, Operation and Care.
- Mixed-Bed Ion Exchange for Complete Protein Capture and Fractionation
- PolyCAT A™ Weak Cation Exchange Technical Sheet
- PolySULFOETHYL® AspartamideSCX Strong Cation Exchange Spec Sheet
- PolyWAX LP® Weak Anion
Exchange, Spec Sheet
- SDS removal
- HIC (Hydrophobic Interaction)
- The Hofmeister Effect and
its implications for HIC. A discussion of the ranking of various ions toward their ability to
retain (precipitate) a mixture of proteins. See also, Douglas J. Tobias and John C. Hemminger, Science, 319, 1197-98 (2008) for a discussion of Specific Ion Effects regarding the hydration of ions on surfaces.
(ALKYL) Aspartamide™ HIC Spec Sheet
HIC Operation and Methods Development. - PolyGLYCOPLEX™ for Complex Carbohydrate Separations, Spec Sheet
- Rapid, Sensitive Combinatorial Library Screening: 10nM Protein-Conjugate Screening by SEC
- SEC Column PolyHYDROXYETHYL Aspartamide Technical Sheet
- Decreasing pH Gradients for Weak Cation Exchange of Peptides
Effect of Organic Solvent on WCX of Proteins
Hemoglobin Analyses by WCX. Comparison of 3µm to 5µm columns for FACS & A1c.
Intact Protein Isolation for Proteomics Bulletin 2006
Monoclonal Antibody Variant Analysis Application Sheet
Protein Variant Analysis Application Sheet
- SCX post ICAT™ and DALPC
fractionation of proteins for Proteomics
Use of pH and salt gradients for 2-D Nano-SCX/RPC-LC/MSMS proteomics fractionation of tryptic digests.
HILIC-SCX of Lung Surfactant Proteins from excess lipids.
Bibliography of PolyLC column references.
PolyLC Part Numbers and Prices
HILIC Methods for Separating Polar Molecules by Polar Differences - Alt.: "Aqueous Normal Phase" (ANP)
- Amino Acids (underivatized) from blood for tissue transplant rejection monitoring
- Comparison of PolyHYDROXYETHYL A® to Amide-80® for small molecule HILIC LC-MS analysis of fermentation broth.
- HILIC for small polar acids, nucleic acid bases and nucleosides. A Comparison (Y. Guo & S. Gaiki, J. Chromatogr. A, 1074 (2005) 71-80.) of Amide, Amine, Silica and betain sulfonate functional groups.
- HILIC, even on raw silica, Increases LC-MS Sensitivity. LCGC article: " Hydrophilic Interaction Chromatography Using Silica Columns for the Retention of Polar Analytes [small molecules sic] and Enhanced ESI-MS Sensitivity"
Amino Acids (underivatized) HILIC LC-MS/MS from plant extracts
Folic Acid Metabolites
General Information and references
Gentamycin (aminoglycoside) antibiotics with LC-MS Detection.
Glucosinolates
Glycopeptides
Glycopeptide Tryptic Mapping , LC-MS
Melamine analysis in food
Metabolomics Profiling
Metabolomics HILIC Comparison to RPC (Tolstikov paper).
Nucleoside Analysis with Volatile Buffers.
Nucleotide Analysis using Electrostatic Repulsion Hydrophilic Interaction (ERLIC).
Phosphorylation Variants of Histone H1.1 HILIC-CEX
Phosphopeptide Isolation using Electrostatic Repulsion Hydrophilic Interaction (ERLIC).
Polar Molecules HILIC
Polar Molecules HILIC LC-MS/MS
Small Molecule applications
Small Molecule HILIC Comparisons
SPE HILIC
- High Capacity HILIC micro-SPE tips
Method Development using HILIC SPE tips
Operating Instructions for HILIC MiniSpin products.
UltraMicroTip columns (tips equivalent to the UltraMicroSpin columns for microliter sample prep).
PolyHYDROXYETHYL™ Aspartamide HILIC Technical Sheet
HILIC Articles (alt: "Aqueous Normal Phase" (ANP)) - HILIC Is Actually Partition Chromatography with Electrostatic and Hydrogen Donor Mechanisms to Utilize for a Separation.
- Bibliography of PolyLC HILIC column references.
Complex Carbohydrate ZIC- HILIC References
HILIC Day, May 31, 2009 10 A.M. - 3:30 P.M. at ASMS Registration.
HILIC Newsletter-1108. Discusses melamine analysis in food products.
HILIC Newsletter-1208. Discusses methyl malonic acid analysis in serum, plasma and urine plus an ELSD detection method for choline and inositol.
HILIC Newsletter-0109. Discusses diethylene glycol analysis in medicine, and suggestions on how to reduce consumption of acetonitrile.
HILIC Newsletter-0309. Discusses alternatives to ACN for HILIC assays and announces the U.S. HILIC Day prior to the ASMS meeting in Philadelphia.
HILIC Review Article: Petrus Hemstrom and Knut Irgum, J. Sep. Sci., 2006, 29, 1784 - 1821. "Hydrophilic Interaction Chromatography."
Hydrophilic interaction liquid chromatography with alcohol as a weak eluent, M. Liu, J. Ostovic, E.X. Chen, N. Cauchon, Journal of Chromatography A (2008), doi:10.1016/j.chroma.2009.01.012.
Retention Time Predictor Tool for ZIC-HILIC columns.
ZIC-HILIC Publications Search
SeQuant® ZIC®-HILIC Columns
-
Applications for Zwitterion HILIC on a Silica Based Betain Sulfonate Surface
- "An HPLC Method for the Determination of Pharmaceutical Salts", LCGC AUG-06: Brian W. Pack and Donald S. Risley
Simultaneous Measurement of Anions & Cations: Method Validation
Poster on use of betain sulfonates bound to silica for Zwitterion HILIC separations
Zwitterion HILIC of cationic peptides
Zwitterion HILIC of glucosinylates, an improved method
Zwitterion HILIC analysis of melamine in food
Zwitterion HILIC of morphine and glucuronated metabolites
Zwitterion HILIC of organophosphonate nerve agent metabolites
Zwitterion HILIC orthogonal method to RPC
Zwitterion HILIC of peptides prior to RPC. 2-D fractionation for proteomics.
Zwitterion HILIC of tryptic peptides
Zwitterion HILIC of homocysteine, methylmalonic acid and succinic acid from biological fluids
-
Applications for Zwitterion pHILIC on a Polymer Based Betain Sulfonate Surface
- ZIC®-pHILIC for Adenosine Phosphate Analysis (AMP, cAMP, dAMP, ADP, dATP and ATP)
Using HILIC Conditions with Volatile Buffers (70% ACN, 30% Ammonium Carbonate, pH 8.8, 100 mM).
Adenosine Phosphate Analysis (AMP, ADP, ATP, CoA, Acetyl-CoA, and Pantothenate) Using HILIC Conditions with Volatile Buffers (80% ACN, 20% 10 mM Ammonium Carbonate, 0.2% NH4OH).
Retention Time Predictor Tool for ZIC-HILIC columns.
ZIC-HILIC Publications
- ZIC-HILIC Part Numbers and Prices
- ZIC®-HILIC & ZIC®-pHILIC General Operational Parameters Before Getting Started:
- ZIC®-HILIC Columns Technical Sheet
ZIC®-pHILIC Polymeric Columns Technical Sheet
ZIC®-HILIC Capillary Columns Technical Sheet
Grace/Vydac®
- Application Notes for Vydac columns
- Vydac Column Care
- Vydac TP (polymeric bonded phase) columns for peptides
- Denali™ C18 (monomeric) C18 for basic and acidic compounds with superior efficiency and reproducibility
-
Best for Small Molecules.
Part Numbers at "238DE™" Prices. - Everest® C18 (monomeric) Ultra High Resolution Protein & Peptide Tryptic Mapping Columns.
- Importance of TFA Concentration for a reproducible peptide separation.
- New! Grace Vydac 0.075 and 0.150mmID Capillary Columns for "Low TFA" LC-MS operation enhanced detection of peptides.
- Mobile Phase modifier effects on bonded phase selectivity and on Low TFA MS bonded silica columns
- Monomeric C18 , 238TP RPC chemistry gives unique selectivities for hydrophobic peptides which elute in the 40-60% MeCN region of the gradient
- Process scale bulk TP and 259VHP medias
- Phospholipid assay with LC-MS or ELSD detection
- Proteomic applications on "low TFA" MS media in prepacked capillary columns from 0.075mmID - 4.6mmID.
- Validation of Reverse Phase HPLC separations (TFA, PO4, HOAc, HCl) Spec. Sheet
- New! Denali™ C18 (monomeric) C18 provides excellent peak symmetry for basic and acidic compounds with superior efficiency and reproducibility for Small Molecules. Prices at "238DE™ Series" Prices.
- Vydac Cross Reference Guide
- Vydac Column Care
-
Comparison of 218TP to PROTO ™ 200 C18 Peptide Columns for 20-30 amino acid peptides.
- Comparison of PROTO300™ C4 to Vydac C4 for proteins.
- Comparison using formic acid to 100Å (LC Packings) column.
Comparison to PROTO™ 200 C18 Peptide Columns for Phosphopeptides.
Part Numbers at "238EV™" Prices.
-
Vydac Part Numbers and Prices
Glycomics and Proteomics Applications:
- Capillary Columns for LC-MS/MS Proteomics Fractionation.
- Comparison of SCX, TiO2, HILIC (HEA) and HILIC (WAX) for Phosphopeptide segregation and/or fractionation.
- HILIC 2-D fractionation of peptides prior to RPC for proteomics.
- SCX pre-columns for post ICAT™ and MudPIT (DALPC) fractionation of proteins for proteomics.
- Use of pH and salt gradients for 2-D Nano-SCX/RPC-LC/MSMS proteomics fractionation of tryptic digests.
- SPE MiniSpin columns and 96-Well Spin Plates for microliter sample prep. For the hardware challenged: Use MiniSpin Tips or UltraMicroSpin SCX columns to fractionate, then load into an autosampler for RPC LC-MS/MS
- Micro filter Page*Eraser™ empty columns remove the particulates from in-gel digests to prolong capillary LC-MS column life
- Mixed-Bed Ion Exchange for Complete Protein Capture and Fractionation
Amino Acid Specific Peptide & Protein Isolation Spin Columns
- BioMolecular Technologies®
Pi3 Methionine
Pi3 Tryptophan
Pi3 Tyrosine Phosphate
Glycomic Separation Techniques
- IP-NPLC [eHILIC] for high mannose and sialylated glycopeptides. "Identification and Quantification of Glycoproteins Using Ion-Pairing Normal-Phase LC and MS," Wen Ding, Harald Nothaft, Christine M. Szymanski, and John Kelly, MCP Papers in Press, June 12, 2009.
HPLC & SPE Proteomic Separation Techniques
DNA & RNA Oligonucleotide Separations:
Higgins Analytical®-
Nucleotide Analysis on Targa C18 5µm 250x4.6mm
column using 15mM ammonium acetate, pH 6.0 with a gradient to 10% MeOH.
Post Transcriptional Modification, Small RNA Nucleotide Separations (C. Callie Coombs, University of Cincinnati, Masters Thesis in Chemistry).
-
Protein and Oligonucleotide separations by HIC or IEX on polymer based columns.
- Nucleogen® for DNA, RNA and Oligonucleotides.
- DEAE-60 Macherey-Nagel Part Numbers and Prices
Oligonucleotide separations on NS 1000 and DEAE-60 columns in volatile buffers.
- DEAE MacroSpin column after PAGE or RPC column separation
- PolyHYDROXYETHYL Aspartamide™ for DeOxy RiboNucleoside Analysis
Using HILIC Conditions with Volatile Buffers.
- ZIC®-pHILIC for Adenosine Phosphate Analysis (AMP, cAMP, dAMP, ADP, dATP and ATP)
Using HILIC Conditions with Volatile Buffers (70% ACN, 30% Ammonium Carbonate, pH 8.8, 100 mM).
Adenosine Phosphate Analysis (AMP, ADP, ATP, CoA, Acetyl-CoA, and Pantothenate) Using ERILIC Conditions with Volatile Buffers (80% ACN, 20% 10 mM Ammonium Carbonate, 0.2% NH4OH).
- Mono-, di- and tri-phosphate Nucleotides on Vydac 302IC SAX columns.
-
Most common TSK-GEL® SEC column and ToyoPearl® media questions
and answers.
Amide-80® Comparison to PolyHYDROXYETHYL A™ for small molecule HILIC LC-MS analysis of fermentation broth
Dialysis:
Simple Dialysis
- Ultra-filtration, Dialysis, Spin Columns or 96 Well Plates for Salt Removal & Buffer Exchange
Simple Dialysis Reusable and Disposable Dialyser - Overview
Reusable Teflon® Biodialyzer® for fast, small volume, salt removal (10µl to 5ml)
Dispo-Biodialyzer® for fast Ultra-Small Volume (10µl to 100µl) salt removal. Disposable Dialysers at a fraction of the cost of slide or cassette products.
Dispo-Biodialyzer® small volume (1-10ml) Disposable Dialysers.
New! 96-Well Dispo-Dialyser. PRICES REDUCED!
Dispo-BioDialyser single unit Instructions
SpinCon™ micro*Dialyser for ultra-small volume (1-5µL) ultra-filtration of samples.
SpinCon Operating Instructions
Flow Through Macro Dialyser for larger volume dialysis is used in conjunction with the Electroeluter-concentrator
On-line micro dialysis for HPLC effluent buffer exchange
Dialyzer Part Numbers & Prices
Equilibrium Dialysis:
- Overview of Reusable dialyzers with replaceable membranes or Disposable Equilibrium Dialysers for Protein or DNA
Binding Studies.
Individual Equilibrium Dispo-Dialyzer®: 75µL disposable kits.
Modular Plate 96-Well Equilibrium Dialyzer™ with individual membranes on 50-200 µL wells. New! ASME robotic dimensioned plates.
96-Well Equilibrium Dialyser Comparison: Old vs. New Specifications.
96-Well Equilibrium Dialyser 8 Plate Rotator picture.
Reusable Two or Three Chamber MicroEquilibrium and FAST Micro-Equilibrium DIALYZERS™
MicroEquilibrium and FAST Micro-Equilibrium DIALYZER™ Instructions
Twenty-cell Multi-Equilibrium Dialyser
Equilibrium Dialysis Formulas and Protocols (Note: 5.7M file using Harvard Apparatus p/n, not Nest p/n)
Equilibrium Dialyzer Part Numbers & Prices
- Dialysis Membrane Conditioning and storage
Dialysis Membrane MSDS data
Dialysis Membrane Overview
Dialysis Membrane Solvent Compatibility
Membrane Part Numbers & Prices
- Bibliography of Equilibrium Dialysis Applications
Equilibrium Dialysis Formulas and Protocols (Note: 5.7M file using Harvard Apparatus p/n, not Nest p/n)
Dialyzer and Equilibrium Dialyzer Prices
Electrophoresis Applications
Agarose for DNA Electrophoresis
- Agarose General Characteristics & Applications
- EXCLU-SIEVE® Agarose: Characteristics, Applications, and Technical Data
- EXCLU-SIEVE™ Part Numbers and Prices
Table Cross Referencing Agarose Products and Applications
Electro-Elution removes a 20-50x molar excess of fluorescent dyes from labelled proteins in 20 minutes
- ElectroPrep Gel Extractor, Protein Concentrator, Excess Dye or SDS Removal Device
- Fast ElectroPrep for 20 minute Removal of Excess Dye from Labelled Protein with Minimal Protein Loss.
Protein Gel Destaining
- Cozap Removes Coomassie Blue From Protein Gels. Crystal clear gels, no blue desk tops, and less expensive to use than tissues or foam. Zap those Coomassie Blues!
- Cozap Part Numbers & Prices
Knitted Open Tubular (KOT) Reactors
Process Columns
Adjustable, Empty Glass Process Columns. An overview of properties and capabilities.Go back to menu.
